Anti-CD244 Rabbit Monoclonal Antibody for WB, FC, ELISA - Q9BZW8

Core Product Specifications and Parameters

Biological Background: CD244 Function and Localization

• CD244 (natural killer cell receptor 2B4, SLAMF4) is a transmembrane receptor of the signaling lymphocytic activation molecule (SLAM) family that binds CD48 as its heterophilic ligand. It modulates immune cell activation and differentiation in both innate and adaptive responses.
• CD244 acts as an activating natural killer (NK) cell receptor, stimulating NK cell cytotoxicity and IFN-gamma production. Related references: PMID:10359122 PMID:11714776 PMID:8376943
• Its activating function involves association with the adapter protein SH2D1A/SAP and kinase FYN, leading to downstream signaling via VAV1, INPP5D/SHIP1, and CBL. Related references: PMID:10934222 PMID:15713798
• CD244 functions as a co-stimulatory receptor, enhancing signals from other NK receptors such as NCR3 and NCR1 to promote NK cell activation. Related references: PMID:10741393
• At early stages of NK cell differentiation, CD244 may act as an inhibitory receptor, potentially ensuring self-tolerance of developing NK cells. Related references: PMID:11917118
• CD244 is expressed on all NK cells, monocytes, basophils, a subset of CD8+ T-cells, and TCR-gamma/delta+ T-cells. Tissue distribution includes spleen, peripheral blood leukocytes, lung, liver, testis, and small intestine.
• The protein localizes to the cell membrane and membrane rafts, consistent with its role in immune synapse signaling.
• CD244 undergoes alternative splicing, yielding isoforms predicted at approximately 30, 36, and 41 kDa, consistent with multiple bands observed by Western blotting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 22-221) of human CD244, targeting the ligand-binding region.
• For Western blotting, consider using reducing conditions and expect multiple bands due to alternative splicing (30, 36, and 41 kDa).
• For flow cytometry, validate staining in human NK cell lines or primary PBMCs, as CD244 is expressed on NK cells, monocytes, basophils, and a subset of T cells.
• For ELISA, pairing with an anti-CD48 antibody may enable detection of receptor-ligand interactions.
• Optimize antibody dilution and incubation conditions in your specific experimental system; the supplied application data serve as a starting point.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a streamlined supply channel for critical IVD raw materials and ancillary reagents. For NK cell and immune receptor research, we provide validated monoclonal antibodies like this anti-CD244 clone, along with complementary reagents such as recombinant CD48 and detection antibodies for ELISA and flow cytometry. Our sourcing platform can also accommodate custom requirements for rare or niche targets in immunology and immuno-oncology.