Anti-CD24 Polyclonal Antibody for WB, IHC-P, ELISA - P25063

Core Product Specifications and Parameters

Biological Background: Signal transducer CD24 Function and Localization

• CD24, also known as Signal transducer CD24 or Small cell lung carcinoma cluster 4 antigen, is a GPI-anchored cell surface glycoprotein encoded by the CD24 gene on human chromosome 6. It is heavily glycosylated and exhibits a small core protein of approximately 8 kDa.

• CD24 plays a pivotal role in cell differentiation across various cell types, with signaling triggered by lectin-like ligand binding to its carbohydrate moieties and transduction via second messengers derived from the GPI anchor.

• In the immune system, CD24 modulates B-cell activation responses; it promotes antigen-dependent proliferation of B-cells and prevents their terminal differentiation into antibody-forming cells. Related references: PMID:11313396

• CD24 associates with SIGLEC10 to selectively suppress immune responses to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90, contributing to the control of autoimmunity.

• The protein is located on the cell membrane and is predominantly expressed on B-cells, T-cells, and various cell lines including erythroleukemia and small cell lung carcinoma, consistent with its role in immune regulation and cancer.

• Keywords associated with CD24 include alternative splicing, cell membrane, glycoprotein, GPI-anchor, lipoprotein, and membrane, highlighting its post-translational modifications and cellular localization.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to amino acids 1-80 of human CD24, which covers the full-length mature protein. This design suggests the antibody likely recognizes both glycosylated and unglycosylated forms, but validation is recommended.
• For Western blotting (WB), consider that CD24’s small size (~8 kDa core) and extensive glycosylation can result in bands migrating higher than expected; optimize SDS-PAGE conditions accordingly.
• This antibody is reported to cross-react with mouse and rat CD24, making it suitable for comparative studies; verify reactivity in each species by testing appropriate positive controls.
• For immunohistochemistry-paraffin (IHC-P), antigen retrieval methods and tissue fixation can affect signal; pilot experiments are advised to establish optimal staining conditions.
• In ELISA applications, confirm binding specificity using recombinant CD24 or cell lysates; pair this antibody with detection reagents compatible with rabbit polyclonal antibodies.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio including validated polyclonal antibodies like anti-CD24. For projects targeting CD24—a key marker in stem cell and immune modulation research—CamelBio can supply antibody pairs, recombinant proteins, and ancillary reagents to streamline your assay development. Contact our team to explore custom procurement or bulk supply options for rare-target raw materials.