Anti-CD239/BCAM Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P50895

Core Product Specifications and Parameters

Biological Background: Basal Cell Adhesion Molecule (BCAM) Function and Localization

• BCAM (Basal Cell Adhesion Molecule), also known as Lutheran blood group glycoprotein (LU), CD239, and Auberger B antigen, is a transmembrane glycoprotein of the immunoglobulin superfamily.
• BCAM serves as a receptor for laminin alpha-5/LAMA5, mediating cell adhesion to the extracellular matrix. Related references: PMID:15975931
• BCAM plays a crucial role in cell motility, migration, and invasion, contributing to tissue organization and dynamics. Related references: PMID:9616226, PMID:31413112
• JAK2-mediated phosphorylation of BCAM activates Rap1/AKT signaling, augmenting laminin adhesion independently of the erythropoietin receptor. Related references: PMID:23160466
• The intracellular domain of BCAM binds to cytoskeletal proteins, including hemoglobin, to mediate signal transduction. Related references: PMID:17158232
• BCAM is expressed on the cell membrane, with highest levels in the pancreas and very low in brain. It is closely associated with the basal layer of epithelial cells and the endothelium of blood vessel walls.
• BCAM features extracellular immunoglobulin domains, multiple disulfide bonds, and N-linked glycosylation sites. Its phosphorylation status modulates adhesion and signaling activity.
• BCAM carries the Lutheran (Lu) blood group antigens and the Auberger B antigen, relevant to immunohematology and transfusion compatibility.

Experimental Guidance and Technical Tips

• Given that the immunogen is a recombinant protein, the antibody likely recognizes multiple epitopes; however, it is advisable to validate performance in your specific assay conditions and sample types.
• For western blot (WB), consider using reducing conditions and load a positive control such as pancreatic or endothelial cell lysates, where BCAM is highly expressed; the expected molecular weight is approximately 67 kDa.
• In immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval may be required; test with known positive tissues like pancreas or blood vessel endothelium alongside appropriate negative controls.
• For ELISA applications, use recombinant BCAM as a reference standard and optimize coating and detection conditions; the monoclonal nature ensures consistent batch-to-batch performance.
• Since cross-reactivity is reported only for human, this antibody may not detect orthologs in other species; verify if your experiments involve non-human samples.
• As BCAM is a cell adhesion molecule, treatment with phosphatase inhibitors during sample preparation may help preserve its phosphorylation state for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and support for rare target procurement. With this anti-CD239/BCAM monoclonal antibody, researchers and diagnostic developers can advance studies in cell adhesion, laminin receptor signaling, cancer biology, and blood group antigen research. Whether you need consistent supply for large-scale production or technical consultation from concept to clinic, CamelBio is equipped to streamline your workflow.