Anti-CD239/BCAM Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P50895

Core Product Specifications and Parameters

Biological Background: CD239/BCAM Function and Localization

• CD239/BCAM (basal cell adhesion molecule) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily, also known as Lutheran blood group glycoprotein, Auberger B antigen, B-CAM, and F8/G253 antigen.
• BCAM functions as a dual receptor and adhesion molecule, critically involved in cell adhesion, motility, migration, and invasion (PubMed:9616226, PubMed:31413112). Related references: PMID:9616226, PMID:31413112
• Its extracellular domain mediates binding to laminin alpha-5 (LAMA5) and other extracellular matrix ligands, while the intracellular domain interacts with cytoskeletal proteins such as hemoglobin to facilitate signal transduction (PubMed:15975931, PubMed:17158232). Related references: PMID:15975931, PMID:17158232
• JAK2 kinase phosphorylates BCAM, which activates a Rap1/AKT signaling cascade to enhance laminin adhesion, demonstrating a key regulatory mechanism independent of erythropoietin receptor (PubMed:23160466). Related references: PMID:23160466
• Localized at the cell membrane, BCAM is widely expressed in tissues, with highest levels in the pancreas and very low expression in brain; it is closely associated with the basal layer of epithelia and the endothelium of blood vessel walls.
• The protein undergoes post-translational modifications including phosphorylation and glycosylation, and is characterized by immunoglobulin-like domains and disulfide bonds, consistent with its classification as a blood group antigen and cell adhesion receptor.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 529–628 of human CD239/BCAM (C-terminal intracellular region). This design supports specificity for the target and compatibility with WB, IHC-P, and ELISA.
• For Western blot, a band around 67 kDa is predicted. Validate using positive controls such as human pancreatic tissue or cell lines (e.g., HEK293, HeLa) under your experimental conditions.
• In IHC-P, perform standard heat-induced antigen retrieval and optimize staining using tissues with known BCAM expression (e.g., pancreas, epithelial basal layer, vascular endothelium).
• Cross-reactivity with mouse suggests potential use in rodent models; however, verification in your specific mouse tissue or cell line is recommended.
• For ELISA, consider pairing with a matched detection antibody for sandwich assay development; antigen coating concentration and buffer conditions may require optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined, one-stop procurement channel for IVD manufacturers and researchers seeking reliable raw materials. This anti-CD239/BCAM rabbit monoclonal antibody exemplifies our commitment to providing validated reagents for cell adhesion and blood group antigen research. Complement your assay development with our broader portfolio, including antibody pairs, bulk ancillary reagents, and custom sourcing services designed to support your project from concept to clinic.