Anti-CD239/BCAM Polyclonal Antibody for WB, ELISA - P50895

Core Product Specifications and Parameters

Biological Background: CD239/BCAM Function and Localization

• CD239/BCAM, also known as basal cell adhesion molecule or Lutheran blood group glycoprotein, is a transmembrane glycoprotein belonging to the immunoglobulin superfamily.
• It functions as a receptor and adhesion molecule mediating cell adhesion, motility, migration, and invasion through interactions with extracellular matrix components and intracellular signaling pathways. Related references: PMID:9616226 PMID:31413112
• The extracellular domain binds laminin, integrin, and other ligands, while the intracellular domain interacts with cytoskeletal proteins such as hemoglobin, facilitating signal transduction. Related references: PMID:17158232
• BCAM serves as a specific receptor for laminin alpha-5 (LAMA5), promoting robust cell adhesion. Related references: PMID:15975931
• JAK2 kinase phosphorylates BCAM and activates its laminin-binding function through a Rap1/AKT signaling pathway, independent of erythropoietin receptor (EPOR). Related references: PMID:23160466
• It is localized to the cell membrane and exhibits wide tissue distribution, with highest expression in the pancreas and very low levels in the brain; it is closely associated with the basal layer of epithelia and the endothelium of blood vessel walls.
• The protein contains immunoglobulin-like domains, disulfide bonds, and multiple phosphorylation sites, and carries the Lu blood group antigens.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 352-547 of human CD239/BCAM, potentially covering regions important for laminin binding; verify epitope accessibility in your assay.
• For WB, consider an initial dilution range of 1:500–1:2000 and include a positive control such as pancreatic tissue lysate or cell lines expressing BCAM.
• Cross-reactivity with mouse allows validation in mouse models; however, optimize conditions for each sample type.
• ELISA applications may require checkerboard titration of both antibody and antigen; use recombinant BCAM protein or cell-based ELISA for protocol development.
• Due to the antibody's polyclonal nature, batch-to-batch consistency should be confirmed for quantitative studies.

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CamelBio offers a comprehensive sourcing platform for IVD raw materials, connecting diagnostic manufacturers and researchers with high-quality reagents like this anti-CD239/BCAM polyclonal antibody. In addition to validated antibodies, we supply optimized antibody pairs, bulk ancillary reagents, and specialize in sourcing rare targets for cell adhesion and oncology research. Contact our team to streamline your procurement and accelerate your assay development.