Anti-CD239/BCAM Monoclonal Antibody for WB, FC, ELISA - P50895

Core Product Specifications and Parameters

Biological Background: CD239/BCAM Function and Localization

• Basal cell adhesion molecule (BCAM, also known as CD239, Lutheran blood group glycoprotein, or Auberger B antigen) is encoded by the BCAM gene (LU, MSK19).
• Functions as both a receptor and an adhesion molecule, playing a crucial role in cell adhesion, motility, migration, and invasion.
  Related references: PMID:9616226 | PMID:31413112
• The extracellular domain binds to extracellular matrix proteins such as laminin and integrin, while the intracellular domain interacts with cytoskeletal proteins like hemoglobin to facilitate cell signal transduction.
  Related references: PMID:17158232
• Acts as a receptor for laminin alpha-5 (LAMA5) to mediate cell adhesion.
  Related references: PMID:15975931
• JAK2 induces BCAM phosphorylation, activating adhesion to laminin via a Rap1/AKT signaling pathway in the absence of erythropoietin receptor (EPOR).
  Related references: PMID:23160466
• Localized to the cell membrane; widely distributed in tissues, with the highest expression in the pancreas and very low in the brain; closely associated with the basal layer of epithelial cells and endothelial cells of blood vessel walls.
• Contains immunoglobulin-like domains and belongs to the immunoglobulin superfamily; classified as a blood group antigen (Lutheran system); post-translationally modified by glycosylation, phosphorylation, and disulfide bond formation.

Experimental Guidance and Technical Tips

• Since the immunogen corresponds to amino acids 32-547 of human CD239/BCAM, the antibody likely targets the extracellular domain, making it suitable for detecting the mature protein on the cell surface in flow cytometry.
• For Western blot, a band near 67 kDa may be expected; consider using whole cell lysates from human tissues with high BCAM expression, such as pancreas.
• In flow cytometry experiments, use appropriate isotype controls and validate the antibody in human cell lines known to express BCAM.
• For ELISA, evaluate performance with recombinant protein or relevant cell lysates to determine optimal coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CD239/BCAM research and diagnostic applications, CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with CamelBio to accelerate your project from development to market.