Anti-CD23 Rabbit Monoclonal Antibody for WB and ELISA - P06734

Core Product Specifications and Parameters

Biological Background: CD23 (FCER2) Function and Localization

• CD23 (FCER2, FCE2, CD23A, IGEBF, CLEC4J, BLAST-2) is a low-affinity receptor for IgE and CR2/CD21, essential for IgE regulation and B-cell differentiation. Related references: PMID:2167225
• On B cells, CD23 mediates IgE-dependent antigen internalization and presentation to T cells. Related references: PMID:2167225
• In macrophages, IgE-bound CD23 triggers cross-linking and activates intracellular parasite killing via the L-arginine-nitric oxide pathway. Related references: PMID:7544003
• CD23 is primarily a membrane protein anchored by a signal-anchor transmembrane helix, but a soluble secreted form is also produced.
• As a member of the C-type lectin family (CLEC4J), it requires calcium for ligand binding and is heavily modified by glycosylation, disulfide bonds, and palmitoylation.
• Protein-level evidence shows CD23 is detectable in urine, consistent with ectodomain shedding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 48-321) of human CD23, encompassing the lectin domain and part of the stalk, making it suitable for detecting both membrane-bound and soluble forms.
• For Western blot, a band near 36 kDa is anticipated; however, due to glycosylation, the observed molecular weight may be higher. Validate detection in B-cell lysates or other IgE-responsive cell models.
• In ELISA, this antibody can be used for direct detection or as part of a matched pair. Consider optimizing coating conditions and testing with recombinant CD23 or native samples (e.g., serum, urine).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with reliable access to IVD-grade antibodies and raw materials. This anti-CD23 rabbit monoclonal antibody supports immunological studies and allergy diagnostics. Our portfolio includes validated antibody pairs, bulk ancillary reagents, and custom sourcing services for rare or emerging targets, streamlining your development workflow.