Anti-CD226/DNAM-1 Monoclonal Antibody for FC - Q8K4F0

Core Product Specifications and Parameters

Biological Background: CD226/DNAM-1 Function and Localization

• CD226, also known as DNAM-1 or PTA1, is a cell surface receptor that plays a critical role in immune intercellular adhesion, lymphocyte signaling, cytotoxicity, and lymphokine secretion by cytotoxic T-cells and NK cells. Related references: PMID:19029379, PMID:24658051, PMID:26552706
• It functions as a costimulatory receptor upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on virus-infected or tumor cells, enhancing the cytotoxic activity of NK cells and CTLs. Related references: PMID:30591568
• Mechanistically, phosphorylation by Src kinases such as LYN or FYN enables binding to the adapter GRB2, leading to activation of VAV1, PI3K, PLCG1, ERK, and AKT, as well as calcium fluxes.
• The protein is localized at the cell membrane and features immunoglobulin domains, a transmembrane helix, disulfide bonds, glycosylation, and phosphorylation sites.
• Also referred to as Platelet and T-cell activation antigen 1, CD226 is involved in platelet and T-cell activation, highlighting its broad role in immune regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 19–254) of mouse CD226/DNAM-1, which may recognize the native receptor on live cells, making it suitable for flow cytometry.
• For FC, titrate the antibody to determine the optimal dilution for your specific cell type and experiment. Consider using appropriate isotype controls.
• As a directly conjugated antibody, no secondary detection reagent is required, reducing background and simplifying multicolor panel design.
• Validate performance in your cell system of interest (e.g., NK cells, T cells), given the reported reactivity with mouse.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with reliable access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This anti-CD226/DNAM-1 antibody supports research in immune cell adhesion and cytotoxicity, and we offer one-stop sourcing for rare target raw materials as well as comprehensive technical support from concept to clinic.