Anti-CD225/IFITM1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P13164

Core Product Specifications and Parameters

Biological Background: CD225/IFITM1 Function and Localization

• CD225/IFITM1 (Interferon-induced transmembrane protein 1) is a 14 kDa transmembrane protein belonging to the dispanin family. It is also known as 9-27, IFI17, LEU13, and DSPA2a.
• As a potent antiviral restriction factor, IFITM1 inhibits entry of diverse viruses including influenza A, SARS-CoV, SARS-CoV-2, Ebola, Marburg, Dengue, West Nile, HIV-1, and HCV by preventing viral fusion after endocytosis. Related references: PMID:26354436 PMID:33270927
• It specifically blocks SARS-CoV-2 spike-mediated cell–cell fusion (syncytia formation). Related references: PMID:33051876
• IFITM1 is implicated in cell adhesion and control of cell growth and migration. Related references: PMID:33270927
• It plays a key role in the antiproliferative action of IFN-gamma by inhibiting ERK activation or inducing p53-dependent G1 arrest.
• IFITM1 acts as a positive regulator of osteoblast differentiation.
• Subcellularly, IFITM1 localizes to the cell membrane and lysosomal membrane.
• Tissue expression: detected in bone, with markedly elevated levels in colon, cervical, esophageal, ovarian cancers and glioma cell lines.
• Post-translational modifications include palmitoylation and phosphorylation, annotated under keywords antiviral defense, innate immunity, osteogenesis, and lipoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–80 of human IFITM1; confirm detection of endogenous full-length protein and evaluate potential cross‑reactivity with IFITM2/3 in your specific assay format.
• For Western blot (expected band ~14 kDa), use high‑percentage Tris‑glycine gels (e.g., 15%) and a PVDF membrane with optimized wet transfer conditions for small proteins.
• In immunofluorescence and immunohistochemistry, include permeabilization steps and appropriate negative controls (isotype control or IFITM1‑knockout cell/tissue) to validate specificity.
• When developing ELISA applications, test the antibody as a capture or detection reagent and verify linearity and sensitivity with recombinant IFITM1 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop platform for IVD raw-material procurement. For anti-CD225/IFITM1 applications in antiviral immunity and oncology, we provide validated monoclonal antibodies, matched antibody pairs, bulk reagents, and custom sourcing for rare targets. Let CamelBio streamline your transition from concept to clinic with reliable supply and expert technical support.