Anti-CD225/IFITM1 Polyclonal Antibody for WB, IHC-P, ELISA - P13164

Core Product Specifications and Parameters

Biological Background: CD225/IFITM1 Function and Localization

• Interferon-induced transmembrane protein 1 (IFITM1, also known as CD225, Leu-13, IFI17, DSPA2a) is a member of the dispanin family and is encoded by the IFITM1 gene.
• Broad-spectrum antiviral restriction factor that inhibits the entry of many enveloped viruses, including influenza A virus, SARS coronaviruses (SARS-CoV and SARS-CoV-2), Ebola virus, HIV-1, and hepatitis C virus. Related references: PMID:26354436 PMID:33270927
• Mechanistically, IFITM1 blocks viral fusion after endocytosis, trapping virions in endosomes and promoting their lysosomal degradation. In hepatocytes, IFITM1 cooperates with IFITM2 and IFITM3 to restrict HCV entry. Related references: PMID:26354436
• Also implicated in cell adhesion, growth control, and migration. IFITM1 mediates the antiproliferative effects of IFN-gamma via p53-dependent cell cycle arrest and inhibits SARS-CoV-2 spike protein-mediated syncytia formation. Related references: PMID:33270927 PMID:33051876
• Localizes primarily to the plasma membrane and lysosome membrane, consistent with its role in blocking viral fusion and directing virions to the degradative pathway.
• Expressed in bone tissue and overexpressed in several cancers, including colon, cervical, esophageal, ovarian, and glioma, making it a potential marker in oncology.
• Post-translationally modified by palmitoylation and phosphorylation; categorized under innate immunity, antiviral defense, and osteogenesis keywords.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 50–125, which should generate antibodies recognizing the full-length protein. Validate reactivity with full-length IFITM1 in interferon-stimulated cell lysates.
• For western blotting, expect a band around 14 kDa. Treatment of cells with type I or type II interferons may upregulate IFITM1 expression.
• In IHC-P, test on tissues known to express IFITM1 (e.g., bone, tumor sections) and optimize antigen retrieval. Use appropriate controls to confirm specificity.
• For ELISA, use the immunizing peptide or recombinant IFITM1 as coating antigen; cross-reactivity with other IFITM family members should be checked.
• Cross-reactivity with mouse allows use in mouse models, but confirm sequence alignment within the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for diagnostic manufacturers and research labs, offering a broad portfolio of IVD raw materials. For CD225/IFITM1 research in antiviral immunity and cancer, we supply validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to streamline your assay development and production. Our sourcing network also enables procurement of rare targets and customized raw material solutions to fit your pipeline needs.