Anti-CD22 Rabbit PolymAb® for WB, IF/ICC, IF-P, IHC-P, ELISA - P20273 / P35329

Core Product Specifications and Parameters

Biological Background: CD22 Function and Localization

• CD22 is a B-cell-specific inhibitory receptor belonging to the sialic acid-binding immunoglobulin-like lectin (siglec) family. It is most highly expressed on B-lymphocytes and plays a role in B-cell differentiation, antigen presentation, and trafficking to bone marrow.
  Related references: PMID:34330755 PMID:8627166
• CD22 binds to alpha 2,6-linked sialic acid residues on surface molecules such as CD22, CD45, and IgM in a cis configuration, and can mediate cell adhesion through trans interactions.
  Related references: PMID:20172905
• As an inhibitory coreceptor, CD22 suppresses B-cell receptor (BCR) signaling by inhibiting calcium mobilization and cellular activation. Phosphorylation of its ITIM domain by LYN kinase recruits the tyrosine phosphatase PTPN6/SHP-1, leading to negative regulation of BCR signaling.
  Related references: PMID:8627166 PMID:20516366
• Strong CD22-mediated inhibitory signaling can induce apoptosis in B-cells.
  Related references: PMID:20516366
• CD22 is localized to the cell membrane as a single-pass type I membrane protein.
• The protein undergoes post-translational modifications including glycosylation, disulfide bond formation, and phosphorylation on tyrosine residues. It contains immunoglobulin-like domains and an ITIM motif essential for its inhibitory function.
• CD22 is exclusively expressed on B-lymphocytes, making it a key marker for B-cell biology and related malignancies.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning human and mouse CD22, suggesting the antibody recognizes conserved epitopes and is suitable for cross-species detection.
• In Western blot (WB), expect bands between 95–97 kDa. Validate the molecular weight against the appropriate cell lysate (e.g., human/mouse B-cell lines) and confirm specificity using positive and negative controls.
• For immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), consider optimizing fixation and permeabilization conditions. The antibody may be compatible with both frozen and paraffin-embedded sections.
• When used in ELISA, test the antibody in a standard sandwich or direct format with recombinant CD22 protein or cell-derived samples.
• Note that functional studies (e.g., blocking assays) may require further validation as the epitope recognized by this PolymAb® is not defined.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop sourcing partner, CamelBio provides diagnostic manufacturers and research institutions with reliable access to high-quality IVD raw materials like this CD22 antibody. Whether you need validated antibody pairs for sandwich immunoassays, bulk monoclonal antibodies for large-scale diagnostic kit production, or ancillary reagents and rare target sourcing, we streamline procurement from concept to market. Our expertise in B-cell biology and immunology helps you accelerate development of in vitro diagnostic solutions.