Anti-CD22 Polyclonal Antibody for WB and ELISA - P20273

Core Product Specifications and Parameters

Biological Background: CD22 Function and Localization

• CD22, also known as SIGLEC2 or BL-CAM, is a member of the sialic acid-binding immunoglobulin-like lectin (siglec) family and is the most highly expressed siglec on B-cells. Related references: PMID:34330755, PMID:8627166
• CD22 functions as an adhesion molecule by binding alpha 2,6-linked sialic acid residues on itself, CD45, and IgM in cis, and also mediates trans interactions with ligands on other cells. Related references: PMID:20172905
• As an inhibitory coreceptor, CD22 negatively regulates B-cell receptor signaling by recruiting the phosphatase PTPN6 via its phosphorylated ITIM domain, leading to inhibition of calcium mobilization and cellular activation. Related references: PMID:8627166
• Upon sufficient negative signaling, CD22 can induce apoptosis in B-cells. Related references: PMID:20516366
• CD22 is a single-pass type I membrane protein localized to the cell membrane.
• It is specifically expressed on B-lymphocytes.
• The protein undergoes post-translational modifications including glycosylation and phosphorylation, and its extracellular region contains immunoglobulin-like domains.
• Alternative splicing of CD22 generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100); consider using this peptide as a positive control in ELISA to confirm antibody reactivity.
• For Western blot, a band near 95 kDa is expected; however, due to glycosylation, the observed molecular weight may be slightly higher. Validate in B-cell lysates.
• This polyclonal antibody may recognize multiple isoforms arising from alternative splicing; assess specificity in your sample system using knockdown or knockout controls if needed.
• Explore B-cell lines such as Raji or Daudi as positive models for both WB and ELISA applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-CD22, and bulk ancillary reagents. Our sourcing capabilities support targets involved in B-cell biology and immune function, facilitating the development of assays for leukemia, lymphoma, and autoimmune disease diagnostics. From concept to clinic, we offer one-stop technical service and consulting to streamline your workflow.