Anti-CD215/IL-15Rα Monoclonal Antibody for IF/ICC, FC - Q60819

Core Product Specifications and Parameters

Biological Background: Interleukin-15 receptor subunit alpha Function and Localization

• Interleukin-15 receptor subunit alpha (IL-15Rα), encoded by the Il15ra gene, is a high-affinity receptor for interleukin-15 (IL-15). Related references: PMID:17947230
• IL-15Rα can signal in both cis and trans configurations; in trans, it presents IL-15 to neighboring cells expressing the IL2RG subunit. Related references: PMID:17947230
• In neutrophils, IL-15Rα binds and activates the kinase SYK upon IL-15 stimulation, and is required for IL-15-induced phagocytosis.
• Subcellularly, IL-15Rα localizes to the cell membrane, nucleus membrane, and cell surface, and is also found as a secreted soluble form in the extracellular space.
• The protein undergoes alternative splicing, glycosylation, phosphorylation, and contains a Sushi domain; it is a transmembrane glycoprotein and a member of the reference proteome.
• Widely expressed across tissues, underscoring its broad role in immune regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region of mouse IL-15Rα (amino acids 33–205), which likely targets the ligand-binding domain. For IF/ICC, consider appropriate fixation and permeabilization methods to detect membrane and nuclear membrane localization.
• For flow cytometry, validate staining in relevant mouse immune cell subsets known to express IL-15Rα.
• As with any antibody, titrate and optimize under your specific experimental conditions to ensure specificity and signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio provides validated antibody pairs, monoclonal antibodies, and ancillary reagents for immunology research. This anti-CD215/IL-15Rα antibody supports studies on IL-15 signaling, with access to bulk purchasing and rare target sourcing. Contact us to discuss your custom requirements and accelerate your diagnostic development from concept to clinic.