Anti-CD209 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NNX6

Core Product Specifications and Parameters

Biological Background: CD209 Function and Localization

• CD209 (DC-SIGN) is a C-type lectin receptor expressed on dendritic cells, alternatively named CLEC4L, CDSIGN, and hDC-SIGN.
• Functions as a pathogen-recognition receptor that mediates endocytosis of pathogens, leading to antigen presentation to T cells and initiation of adaptive immunity.
• Serves as a high-affinity receptor for ICAM2 and ICAM3, facilitating dendritic cell rolling, transendothelial migration, and T-cell clustering, thereby promoting T-cell proliferation. Related references: PMID:10721994 PMID:10721995 PMID:11017109
• Acts as an attachment receptor for a wide range of viruses, including HIV-1, Ebola virus, HCV, Dengue virus, SARS-CoV, and Lassa virus (PubMed:23966408), facilitating viral entry into host cells. Related references: PMID:23966408
• Recognizes bacterial and parasitic pathogen antigens, including mannose-capped lipoarabinomannan (ManLAM) from Mycobacterium tuberculosis and Lewis-x-containing glycans from Helicobacter pylori and Schistosoma mansoni, playing a role in innate immune recognition. Related references: PMID:16092920 PMID:21203928 PMID:16379498
• Localizes primarily to membrane rafts and the plasma membrane, but also exists in secreted forms, consistent with its roles in cell adhesion and pathogen capture.
• Predominantly expressed in dendritic cells and DC-rich tissues, as well as placental macrophages, endothelial cells of placental vascular channels, and peripheral blood mononuclear cells.

Experimental Guidance and Technical Tips

• When performing western blot, a band near the predicted 46kDa should be detected; we recommend optimizing sample preparation with appropriate lysis buffers.
• In immunofluorescence/ICC, permeabilization may be required to detect intracellular CD209; consider using dendritic cell lines or primary cells as positive controls.
• For ELISA applications, pairing with a suitable capture/detection antibody is advised; test conditions using recombinant CD209 protein.
• Given cross-reactivity with mouse and rat, the antibody may be used for studies in rodent models, but validation in the relevant species is essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including this anti-CD209 rabbit polyclonal antibody, ideal for infectious disease and immunology research. As your dedicated sourcing partner, we offer validated antibody pairs, bulk ancillary reagents, and customized solutions to accelerate your diagnostic development from concept to clinic.