Anti-CD209 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9NNX6

Core Product Specifications and Parameters

Biological Background: CD209 Function and Localization

• CD209, also known as DC-SIGN, CLEC4L, or CDSIGN, is a C-type lectin receptor predominantly expressed on the surface of immature dendritic cells.
• It functions as a pathogen-recognition receptor, mediating endocytosis of pathogens and subsequent antigen presentation to T cells via MHC class II molecules, thus linking innate and adaptive immunity.
• As a high-affinity receptor for ICAM2 and ICAM3, CD209 facilitates dendritic cell rolling and transendothelial migration, and forms a first contact between DCs and resting T cells through ICAM3 binding, promoting T cell proliferation. Related references: PMID:10721994 PMID:10721995 PMID:11017109
• CD209 serves as an attachment receptor for numerous viruses, including HIV-1, HIV-2, Ebolavirus, HCV, Dengue virus, SARS-CoV, and Lassa virus, facilitating viral entry into host cells. Related references: PMID:23966408
• In bacterial infections, CD209 recognizes high-mannose N-linked oligosaccharides on pathogens such as Mycobacterium tuberculosis and Helicobacter pylori, contributing to host defense. Related references: PMID:16379498 PMID:16092920 PMID:21203928
• Subcellularly, CD209 localizes primarily to membrane rafts and the cell membrane, but secreted isoforms also exist; it is highly expressed in dendritic cells, placental macrophages, and certain endothelial cells.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1–100 near the N-terminus, which suggests the antibody may detect both native and denatured forms, supporting applications such as WB, IHC-P, IF/ICC, and ELISA.
• For Western blotting, a band around 46 kDa is expected; validate with appropriate positive control lysates and consider using a reducing SDS-PAGE gel.
• In IHC-P on formalin-fixed paraffin-embedded tissues, antigen retrieval may improve staining; optimize the retrieval method and antibody dilution for your specific samples.
• For IF/ICC, permeabilization is required to access the intracellular epitope; use a suitable permeabilization buffer and confirm specificity with negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. This CD209 monoclonal antibody supports immune response and infectious disease research, and we can assist with bulk sourcing or customized solutions to meet your project requirements. With one-stop access from concept to clinic, CamelBio is your reliable partner for rare target raw materials and technical consulting.