Anti-CD204 (MSR1/SCARA1) Rabbit Monoclonal Antibody for IHC-P, FC, ELISA - P21757

Core Product Specifications and Parameters

Biological Background: CD204 Function and Localization

• CD204 (MSR1/SCARA1) is a macrophage scavenger receptor that mediates binding, endocytosis, and phagocytosis of modified low-density lipoproteins (acetylated and oxidized LDL), promoting cholesterol uptake and foam cell formation during atherogenesis. Related references: PMID:2251254, PMID:34252459
• It recognizes pathogen-associated molecular patterns and damaged‑associated molecules, including bacterial cell wall components and apoptotic debris, thereby supporting innate immune clearance. Related references: PMID:10722588
• Upon CpG DNA stimulation, CD204 activates LYN kinase, which phosphorylates SYK, leading to enhanced endosomal TLR9 signaling and cytokine production. Related references: PMID:29057947, PMID:40980882
• Three major isoforms (I, II, III) arise from alternative splicing; isoform I and II are detected in monocyte‑derived macrophages, liver, placenta, and brain, while isoform III is macrophage‑restricted.
• The protein contains coiled‑coil, collagen‑like, and transmembrane domains and is subject to N‑linked glycosylation, resulting in mature forms migrating at ~40–50 kDa.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 77–451 of the extracellular region, making the antibody suitable for detecting native CD204 on the cell surface in IHC‑P and flow cytometry.
• For ELISA, consider pairing this antibody with a non‑competing detector or using recombinant CD204 protein as a standard to establish assay sensitivity.
• Multiple protein bands (40/43/50 kDa) may be observed due to isoform variation and glycosylation; validate the band pattern in the relevant human macrophage cell line or tissue lysate.
• Since cross‑reactivity is reported only for human, we recommend testing reactivity with other species if required for multi‑species studies.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio streamlines IVD raw‑material procurement for diagnostic manufacturers and research laboratories, offering this anti‑CD204 antibody as part of a comprehensive portfolio for atherosclerosis and innate immunity research. Beyond single antibodies, we supply validated antibody pairs, bulk monoclonals and polyclonals, and ancillary reagents to support assay development. Should your program require rare‑target raw materials or custom sourcing, our team acts as a responsive bridge from concept to clinic.