Anti-CD204 Monoclonal Antibody for Flow Cytometry - P21757

Core Product Specifications and Parameters

Biological Background: CD204 (MSR1) Function and Localization

• CD204, also known as macrophage scavenger receptor types I and II (MSR1) or scavenger receptor class A member 1 (SCARA1), is a transmembrane glycoprotein that exists in multiple isoforms generated by alternative splicing (Isoform I, II, III).
• It mediates the binding and endocytosis of modified low-density lipoproteins such as acetylated LDL and oxidized LDL, facilitating cholesterol uptake and contributing to foam cell formation during atherogenesis. Related references: PMID:2251254, PMID:34252459
• CD204 recognizes a wide array of pathogen-associated and damage-associated molecular patterns, including bacterial cell wall components and apoptotic cell debris, enabling macrophage clearance functions in innate immunity. Related references: PMID:10722588
• Upon CpG DNA stimulation, CD204 activates LYN kinase, which phosphorylates SYK, leading to recruitment to endosomal TLR9 and enhancement of TLR9 signaling. Related references: PMID:29057947, PMID:40980882
• The protein is primarily localized to the cell membrane, with expression in monocyte-derived macrophages and in tissues such as liver, placenta, and brain.
• Key structural features include a signal-anchor, transmembrane helix, coiled-coil domains, and collagen-like regions; it is glycated and phosphorylated.
• Different isoforms exhibit functional variations; for example, one isoform does not internalize acetylated LDL.

Experimental Guidance and Technical Tips

• For flow cytometry, consider using a live-cell staining protocol to detect surface CD204 expression on macrophages. Optimize antibody titration and include appropriate isotype controls to ensure specificity.
• Since the antibody is rabbit monoclonal, it may provide consistent batch-to-batch performance. Validate the staining in your specific experimental system, especially when analyzing rare cell populations.
• Prepare single-cell suspensions from human PBMCs or tissue-derived macrophages, and use a viability dye to exclude dead cells. The conjugate is compatible with standard flow cytometers equipped with a 561 nm or 594 nm laser.
• CD204 expression can be modulated by certain stimuli; consider testing both resting and activated macrophages to confirm detection across activation states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, providing diagnostic manufacturers and researchers with validated antibodies, bulk reagents, and custom sourcing for rare targets like CD204. Our portfolio supports atherosclerosis and innate immunity research with ready-to-use conjugated antibodies and ancillary reagents. From concept to clinic, CamelBio streamlines procurement with supply chain flexibility and technical support.