Anti-CD200 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P41217

Core Product Specifications and Parameters

Biological Background: CD200 Function and Localization

• CD200, also known as OX-2 membrane glycoprotein or MOX1/MOX2, is a transmembrane protein belonging to the immunoglobulin superfamily.
• It functions as a costimulatory molecule for T-cell proliferation and may regulate myeloid cell activity in various tissues.
• Encoded by the CD200 gene, this protein undergoes alternative splicing, generating multiple isoforms.
• Subcellular localization: Predominantly expressed on the cell membrane, consistent with its role in cell-cell interactions.
• The protein contains an immunoglobulin domain, a signal peptide, and a transmembrane helix, along with disulfide bonds and glycosylation sites.
• Keywords include alternative splicing, cell membrane, disulfide bond, glycoprotein, immunoglobulin domain, signal, transmembrane, and reference proteome, indicating its structural and functional characteristics.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 31–232 of human CD200 (extracellular domain after signal peptide), suggesting suitability for detecting both native and recombinant CD200.
• For Western blot (WB), expect a band corresponding to the predicted molecular weight of approximately 31 kDa under reducing conditions.
• For immunohistochemistry (paraffin-embedded sections, IHC-P), antigen retrieval conditions should be optimized for expected expression in relevant tissues.
• For ELISA, the antibody can be used as capture or detection reagent; titration is recommended to determine optimal working concentration.
• Since cross-reactivity is indicated only for human, verify reactivity in other species if applicable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop bridge for IVD raw materials, connecting diagnostic manufacturers with high-quality reagents. This CD200 monoclonal antibody is part of our expanding portfolio for immune-related targets, supporting research in immunoregulation and T-cell biology. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development from concept to clinic.