Anti-CD200 Polyclonal Antibody for WB, ELISA - O54901

Core Product Specifications and Parameters

Biological Background: CD200 Function and Localization

• CD200, also known as OX-2 membrane glycoprotein or MRC OX-2 antigen, is a cell surface glycoprotein belonging to the immunoglobulin superfamily.
• It functions as a costimulatory molecule for T-cell proliferation, providing a regulatory signal that may influence immune response thresholds.
• CD200 may also regulate myeloid cell activity across diverse tissues, contributing to immune homeostasis (By similarity).
• The protein localizes to the cell membrane and contains a single transmembrane helix, along with an N-terminal signal peptide.
• Post-translational modifications include N-linked glycosylation and at least one disulfide bond within its immunoglobulin-like domain.
• The UniProt record classifies CD200 as a reference proteome member with a known 3D structure, underscoring its role as a fundamental immune regulator.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 31–232 of mouse CD200, covering the extracellular region, which may favor recognition of the native surface protein.
• For Western blot, use reducing conditions and run a positive control such as mouse spleen or thymus lysate; the expected band is approximately 31 kDa.
• ELISA applications may be developed using recombinant CD200 protein as a standard; validate antibody performance against relevant mouse or rat tissue samples.
• Consider species cross-reactivity with rat when designing experiments, as indicated by the supplier; preliminary testing in the target rat model is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers and laboratories as a one-stop partner for IVD raw materials, from concept to clinic. This anti-CD200 polyclonal antibody supports immunoassay development in immune checkpoint and myeloid cell regulation research. We offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets, simplifying your supply chain and accelerating product development.