Anti-CD20 Rabbit Monoclonal Antibody for WB and IHC-P - P11836

Core Product Specifications and Parameters

Biological Background: CD20 Function and Localization

• B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Related references: PMID:12920111, PMID:3925015, PMID:7684739
• Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR. Related references: PMID:12920111, PMID:18474602, PMID:7684739
• Localized to the cell membrane, with expression specific to B-cells.
• Contains key post-translational modifications including palmitoylation, phosphorylation, and a disulfide bond; it is also classified as a lipoprotein.
• A well-established pharmaceutical target for B-cell depletion therapies, highlighted by its inclusion in the UniProt pharmaceutical keyword.
• Alternative splicing generates multiple isoforms of the protein.
• The canonical isoform has a molecular weight of approximately 33 kDa (UniProt annotated mass: 33,077 Da).

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide mapping to amino acids 100–200 of human CD20, a region that may include extracellular epitopes suitable for recognizing native surface antigen.
• For western blot (WB), a band near 33 kDa is anticipated in B-cell lysates; consider running appropriate positive controls (e.g., Raji or Daudi cell lines) and validating with siRNA or knockout models.
• In immunohistochemistry (IHC-P), CD20 staining is expected to be membrane-localized in B-cell zones of lymphoid tissues (e.g., tonsil, lymph node); optimize antigen retrieval and antibody titration for formalin-fixed paraffin-embedded sections.
• For flow cytometry (FC) and immunofluorescence (IF/ICC), test on B-cell lines or primary PBMCs; confirm specificity using isotype controls and verify cross-reactivity with cynomolgus monkey as stated.
• ELISA applications may require pairing with a suitable detection antibody; consider performing checkerboard titrations and addressing potential matrix effects from serum or plasma samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material procurement, offering a comprehensive portfolio from validated antibody pairs to bulk ancillary reagents. For researchers and diagnostic developers focusing on B-cell malignancies or immune profiling, our CD20 monoclonal antibody serves as a reliable building block in lymphoma panel development, backed by CamelBio’s rapid sourcing and technical consultative support. Whether you need consistent lot-to-lot performance, customized formulations, or access to rare targets, CamelBio bridges the gap from concept to clinic.