Anti-CD20 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P11836

Core Product Specifications and Parameters

Biological Background: CD20 Function and Localization

• CD20 (MS4A1) is a B-lymphocyte-specific membrane protein that plays a role in regulating calcium influx, which is essential for B-cell development, differentiation, and activation. Related references: PMID:12920111, PMID:3925015, PMID:7684739
• It functions as a store-operated calcium (SOC) channel component, promoting calcium influx after B-cell receptor (BCR) activation. Related references: PMID:12920111, PMID:18474602, PMID:7684739
• CD20 is exclusively localized to the cell membrane and contains multiple transmembrane domains.
• Expression is highly restricted to B-cells, making it a key marker for B-lymphocyte lineages.
• It belongs to the membrane-spanning 4-domains subfamily A and is also known as Bp35 or Leu-16.
• The protein undergoes post-translational modifications including disulfide bond formation, palmitoylation, and phosphorylation, which may modulate its function.
• Alternatively spliced variants exist, potentially giving rise to different isoforms.
• CD20 is a clinically important pharmaceutical target, particularly in B-cell malignancies and autoimmune disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the intracellular C‑terminal region (aa 197‑297) of human CD20, suggesting the antibody primarily detects the cytoplasmic domain.
• For Western blot (WB), expect a band around 33–37 kDa; the migration may shift slightly due to post‑translational modifications.
• In immunohistochemistry (IHC-P), B‑cell‑rich tissues such as lymph node or spleen serve as ideal positive controls; include appropriate negative controls.
• For immunofluorescence (IF/ICC), permeabilization is likely required to access the intracellular epitope.
• When using ELISA, validate with recombinant CD20 or cell lysates from a B‑cell line to confirm reactivity.
• As a polyclonal antibody, lot‑to‑lot consistency should be confirmed in the relevant assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs streamlined access to IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare‑target antibodies like this anti‑CD20 polyclonal antibody. With expertise spanning the CD20/B‑cell research area, CamelBio can support your procurement of optimized monoclonal/polyclonal antibodies and related reagents, helping you move from concept to clinic efficiently.