Anti-CD2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P06729

Core Product Specifications and Parameters

Biological Background: CD2 Function and Localization

• CD2, also known as T-cell surface antigen CD2, LFA-2, and the rosette receptor, is a cell adhesion molecule of the immunoglobulin superfamily.
• The protein mediates adhesion between T-cells and other cell types by interacting with lymphocyte function-associated antigen 3 (LFA-3/CD58) and CD48/BCM1, playing a critical role in immune synapse formation and T-cell activation.
• CD2 is implicated in T-cell triggering through its cytoplasmic domain, which contributes to signal transduction pathways essential for T-cell responses.
• The extracellular region contains immunoglobulin-like domains, and the protein is anchored to the cell membrane by a single-pass transmembrane helix.
• CD2 is a glycoprotein with disulfide bonds and N-linked glycosylation, which are important for its structural stability and ligand interaction.
• It is expressed on most T-cells and natural killer (NK) cells, with protein-level detection confirmed in NK cells, highlighting its role in both adaptive and innate immunity.
• UniProt keywords include cell adhesion, immunoglobulin domain, repeat, glycoprotein, and disulfide bond, reflecting its modular structure and adhesive function.

Experimental Guidance and Technical Tips

• For Western blot (WB) analysis, an expected band near 39-40 kDa can be observed; validate in a relevant sample system such as Jurkat T-cell lysates.
• For immunohistochemistry paraffin sections (IHC-P), consider appropriate antigen retrieval and antibody dilution; tissue microarrays with lymphoid tissues may serve as positive controls.
• In ELISA applications, this antibody may be used for capture or detection of recombinant or endogenous CD2; pre-testing with known CD2-expressing cell lysates is recommended.
• Given the immunogen is a recombinant protein of human CD2, the antibody likely recognizes the extracellular domain; confirm specificity with peptide competition or knockdown experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CD2-targeted research in immunology and T-cell biology, we offer validated antibody pairs, optimized rabbit monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing support for rare target raw materials. Whether prototyping an immunoassay or scaling production, our integrated supply chain helps streamline your development.