Anti-CD2 Rabbit Monoclonal Antibody for WB, FC, ELISA - P08920

Core Product Specifications and Parameters

Biological Background: CD2 Function and Localization

• CD2 (T-cell surface antigen CD2) is also known as LFA-2, LFA-3 receptor, lymphocyte antigen 37 (Ly-37), and T11/Leu-5. It is a member of the immunoglobulin superfamily, characterized by extracellular immunoglobulin-like domains.
• The protein functions as a cell adhesion molecule, interacting with CD58 (LFA-3) and CD48/BCM1 on antigen-presenting cells and other targets to mediate intercellular adhesion between T-cells and other cell types.
• Beyond adhesion, CD2 is implicated in T-cell activation and signaling; its cytoplasmic domain is critical for triggering signaling pathways that modulate T-cell responses.
• Structurally, CD2 is a single-pass type I transmembrane glycoprotein with an extracellular region containing two immunoglobulin-like domains, a transmembrane helix, and a cytoplasmic tail involved in signal transduction.
• Subcellularly, CD2 is localized at the cell membrane, consistent with its role in cell–cell interactions and surface receptor functions.
• In mice, Cd2 is detected in thymus and spleen tissues, aligning with its expression in T-lymphoid lineages.
• Keywords from UniProt highlight additional features: disulfide bond, glycoprotein, immunoglobulin domain, repeat, signal peptide, transmembrane helix, and cell adhesion molecule.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 23-203) of mouse CD2, which may confer specificity toward surface-expressed antigen in native and denaturing conditions.
• For Western blot (WB), the predicted protein weight is ~38 kDa; consider including appropriate positive controls such as mouse splenocyte or thymocyte lysates.
• In flow cytometry (FC), use of live, unfixed cells may preserve epitope conformation; titration of antibody is recommended to optimize staining index.
• ELISA applications may benefit from recombinant CD2 protein or peptide coating; cross-reactivity should be verified with relevant species samples if extending beyond mouse.
• As a rabbit monoclonal antibody, lot-to-lot consistency is expected, but routine validation in the specific sample system is advisable.

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CamelBio streamlines IVD raw material procurement for diagnostic manufacturers, labs, and research institutes, providing high-quality monoclonal antibodies like this anti-CD2 rabbit monoclonal antibody for T-cell adhesion and immunology research. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing support—ensuring you can move from concept to clinic with confidence.