Anti-CD2 Monoclonal Antibody for WB, FC, ELISA - P06729

Core Product Specifications and Parameters

Biological Background: CD2 Function and Localization

• CD2 is a T-cell surface antigen also known as T11, SRBC, LFA-2, and the rosette receptor.
• It functions as a cell adhesion molecule by interacting with CD58 (LFA-3) and CD48/BCM1, mediating adhesion between T cells and antigen-presenting cells or target cells.
• The cytoplasmic domain of CD2 is involved in signal transduction, contributing to T-cell activation and triggering.
• CD2 is a glycoprotein with an extracellular region containing two immunoglobulin-like domains and a disulfide bond, classifying it as a member of the immunoglobulin superfamily.
• It is localized at the cell membrane as a single-pass type I transmembrane protein.
• At the protein level, CD2 is expressed in natural killer cells, in addition to T cells.
• Post-translational modifications include N-linked glycosylation, and the protein has a predicted molecular weight of approximately 39.4 kDa.

Experimental Guidance and Technical Tips

• For Western blot (WB), the target protein has a predicted molecular weight of ~39-40 kDa. Consider using appropriate reducing conditions and validate band specificity with positive control cell lysates.
• For flow cytometry (FC), CD2 is a cell surface marker. Use standard staining protocols with appropriate cell populations (e.g., T cells, NK cells) and verify by co-staining with established markers.
• In ELISA, the recombinant immunogen (aa 25-209) corresponds to the extracellular domain; consider pairing with a detection antibody targeting a non-overlapping epitope for sandwich ELISA.
• The antibody is raised against human CD2; cross-reactivity with other species has not been tested, so validate in the relevant sample system if used beyond human.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material sourcing by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare targets. This anti-CD2 monoclonal antibody supports immunology and infectious disease research applications. Our one-stop supply chain ensures seamless procurement from concept to clinic.