Anti-CD1D Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P15813

Core Product Specifications and Parameters

Biological Background: CD1D Function and Localization

• CD1D (R3G1) is an antigen-presenting glycoprotein of the CD1 family, primarily involved in presenting lipid antigens to natural killer T (NKT) cells.
• It binds self and non-self glycolipids within its hydrophobic binding groove and presents them to T-cell receptors on NKT cells, bridging innate and adaptive immunity.
• CD1D is expressed on cortical thymocytes, certain T-cell leukemias, and in a variety of other tissues, supporting its role in immune surveillance.
• The protein localizes to the cell membrane, basolateral cell membrane, endosome membrane, lysosome membrane, and endoplasmic reticulum membrane, reflecting its trafficking through the endocytic pathway for antigen loading.
• Post‑translational modifications include glycosylation and disulfide bond formation, contributing to its structural stability and ligand‑binding function.
• The predicted molecular weight of CD1D is approximately 37.7 kDa, though glycosylation may result in a higher apparent molecular weight (observed at 38 kDa in some systems).

Experimental Guidance and Technical Tips

• For Western blot, consider loading appropriate positive controls such as lymphoid cell lines (e.g., Jurkat) and using reducing loading buffer to resolve the ~38 kDa band.
• When performing immunofluorescence, permeabilize cells with 0.1% Triton X-100 to detect intracellular pools of CD1D.
• Since the antibody cross-reacts with mouse, validate its specificity in your model system by using CD1D knockout or knockdown controls.
• ELISA applications may require optimization of coating antigen and detection conditions.
• This polyclonal antibody was raised against the extracellular domain (aa 20-190); thus, it may detect both membrane-bound and soluble forms of CD1D.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supplying validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This CD1D antibody supports research into innate immunity and glycolipid antigen presentation. Reach out for technical services and tailored procurement solutions from concept to clinic.