Anti-CD1d Monoclonal Antibody for WB, IF/ICC, FC, ELISA - P15813

Core Product Specifications and Parameters

Biological Background: CD1d Function and Localization

• CD1d is an antigen-presenting glycoprotein belonging to the CD1 family of MHC-like molecules, also known as R3G1.
• It binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells, playing a key role in innate immunity.
• Subcellular localization includes the cell membrane, basolateral cell membrane, endosome membrane, lysosome membrane, and endoplasmic reticulum membrane, consistent with its antigen-processing and presentation function.
• Tissue expression is observed on cortical thymocytes, certain T-cell leukemias, and in various other tissues.
• Post-translational modifications include disulfide bond formation and glycosylation, typical of immunoglobulin domain-containing membrane proteins.
• Structural features include a signal peptide, an immunoglobulin domain, a transmembrane helix, and a short cytoplasmic tail.

Experimental Guidance and Technical Tips

• For Western blot (WB), CD1d has a predicted molecular weight of ~38 kDa; consider using reducing conditions and validate with a positive control such as human thymocyte lysate.
• Immunofluorescence/immunocytochemistry (IF/ICC) may detect surface and endosomal localization; fixation and permeabilization conditions should be optimized for each cell type.
• Flow cytometry (FC) is suitable for detecting cell surface CD1d on live cells; titrate antibody concentration and include appropriate isotype controls.
• For ELISA, validate the antibody in direct or capture formats using recombinant CD1d or cell lysates; assay conditions require empirical optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes worldwide as a comprehensive supplier of IVD raw materials. This anti-CD1d monoclonal antibody fits into immunology and innate immunity research, and we can source validated antibody pairs, optimized antibodies, bulk ancillary reagents, and rare-target raw materials to accelerate your assay development projects.