Anti-CD1B Rabbit Monoclonal Antibody for IF/ICC, FC, ELISA - P29016

Core Product Specifications and Parameters

Biological Background: CD1B Function and Localization

• CD1B is a T-cell surface glycoprotein belonging to the CD1 family of antigen-presenting molecules.
• It binds and presents self and non-self lipid and glycolipid antigens to natural killer T-cell receptors, playing a crucial role in adaptive immunity.
• The protein localizes to the cell membrane, endosome membrane, and lysosome membrane, consistent with its function in lipid antigen trafficking and presentation.
• Expressed on cortical thymocytes, certain T-cell leukemias, and various other tissues, highlighting its relevance in T-cell development and leukemia.
• Structural features include an immunoglobulin domain, disulfide bonds, and glycosylation sites, which contribute to its stability and immune recognition.
• Alternative splicing produces multiple isoforms, with observed molecular weights around 37 kDa and 30 kDa, as indicated by the product data.
• Keywords associated with CD1B include adaptive immunity, membrane, glycoprotein, and disulfide bond, underscoring its role in immune system processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 18-303), suggesting suitability for detecting native CD1B in immunofluorescence and flow cytometry applications.
• For flow cytometry, consider using live cells to target cell surface CD1B, while permeabilization may be required for intracellular or endosomal/lysosomal populations.
• In immunofluorescence assays, validate fixation and permeabilization conditions that preserve epitope recognition, and always include appropriate controls in the relevant human sample system.
• ELISA reactivity can be explored by coating recombinant CD1B or cell lysates; confirm specificity with known positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research laboratories, offering validated antibody pairs, bulk-scale monoclonal/polyclonal antibodies, and ancillary reagents. For CD1B-related immunology research, we can support your entire workflow—from concept to clinic—with reliable, quality-controlled materials. Contact us to discuss rare-target sourcing and customized supply solutions tailored to your development needs.