Anti-CD19 Rabbit Monoclonal Antibody for WB, ELISA - P15391

Core Product Specifications and Parameters

Biological Background: CD19 Function and Localization

• CD19 acts as a coreceptor for the B-cell antigen receptor (BCR) complex on B-lymphocytes, lowering the threshold for activation of downstream signaling and enhancing B-cell responses to antigens.
  Related references: PMID:29523808 PMID:1373518 PMID:16672701 PMID:2463100

• It activates phosphatidylinositol 3-kinase signaling and mobilizes intracellular Ca²⁺ stores upon BCR engagement.
  Related references: PMID:12387743 PMID:16672701 PMID:9317126

• CD19 is not required for early B cell differentiation in the bone marrow but is essential for normal B cell differentiation and proliferation following antigen challenge.
  Related references: PMID:9317126 PMID:1373518 PMID:2463100

• It plays a critical role in maintaining normal serum immunoglobulin levels and in generating high-affinity antibodies during immune responses.
  Related references: PMID:12387743 PMID:16672701 PMID:9317126

• The protein is localized to the cell membrane and enriched in membrane rafts.

• CD19 is detected on marginal zone and germinal center B cells in lymph nodes, as well as on circulating blood B cells.

• Post-translational modifications include glycosylation and phosphorylation; the protein contains immunoglobulin domains, disulfide bonds, and a transmembrane helix.

• Alternative splicing produces multiple isoforms of CD19, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen used is recombinant full-length human CD19 protein; thus, the antibody likely recognizes the native folded structure.
• For Western blotting, a band around 61kDa is expected; reducing conditions should be evaluated.
• Cross-reactivity with mouse and rat CD19 is reported; verify performance in the relevant species and sample type (e.g., B-cell lines or primary lymphocytes).
• For ELISA, the antibody may be used as a capture or detection reagent; optimal working dilutions should be determined by titration.
• Because CD19 expression is B-cell–specific, ensure proper positive and negative controls (e.g., B-cell vs. non-B-cell lysates).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, bridging the gap from early-stage research to clinical diagnostics. This anti-CD19 rabbit monoclonal antibody exemplifies our commitment to providing high-quality reagents for B-cell immunophenotyping and diagnostic assay development. Beyond single antibodies, we supply validated antibody pairs, bulk ancillary reagents, and rare-target sourcing to keep your pipeline moving efficiently.