Anti-CD19 Polyclonal Antibody for WB, IF/ICC, ELISA - P15391

Core Product Specifications and Parameters

Biological Background: CD19 Function and Localization

• CD19 is a B-lymphocyte antigen (also known as B4 or Leu-12) that functions as a co-receptor for the B-cell receptor (BCR) complex, essential for modulating B-cell signaling thresholds. Related references: PMID:29523808
• It lowers the antigen concentration required for B-cell activation, thereby enhancing sensitivity to low-abundance antigens and promoting robust immune responses. Related references: PMID:1373518 PMID:16672701 PMID:2463100
• CD19 activates downstream signaling cascades, notably the PI3K–AKT pathway, and triggers intracellular calcium mobilization, critical for B-cell proliferation and differentiation. Related references: PMID:12387743 PMID:16672701 PMID:9317126
• While dispensable for early B-cell development in the bone marrow, CD19 is indispensable for antigen-driven B-cell proliferation, immunoglobulin production, and high-affinity antibody maturation. Related references: PMID:9317126 PMID:1373518 PMID:2463100 PMID:12387743 PMID:16672701
• Located on the cell membrane and enriched in lipid rafts, its precise membrane localization is vital for efficient signaling.
• Expressed on marginal zone and germinal center B cells in lymph nodes, as well as on peripheral blood B cells, underscoring its role in humoral immunity. Related references: PMID:2463100 PMID:16672701
• Post-translational modifications include phosphorylation and glycosylation; it is a transmembrane glycoprotein with immunoglobulin-like domains and disulfide bonds, contributing to its stability and receptor interactions.

Experimental Guidance and Technical Tips

• For Western blot, a band at approximately 61 kDa is expected. Optimize antibody dilution and use appropriate blocking conditions. Confirm specificity with positive and negative control lysates.
• In immunofluorescence/immunocytochemistry, the intracellular epitope (amino acids 457–556) requires permeabilization. Fix cells appropriately and titrate the antibody to reduce background.
• For ELISA, perform titration experiments to determine optimal coating and detection concentrations. Validate with known CD19-positive and -negative samples.
• Due to cross-reactivity with mouse and rat, this antibody is suitable for comparative studies across species. However, verify reactivity in your specific tissue or cell line of interest.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio streamlines your procurement of validated antibodies, bulk reagents, and rare-target raw materials. Our CD19 polyclonal antibody supports B-cell immunology research and diagnostic development, offering consistent quality and supply chain efficiency. Contact us to explore custom bulk options and complementary reagent packages tailored to your project needs.