Anti-CD18 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P05107

Core Product Specifications and Parameters

Biological Background: CD18 Function and Localization

• CD18 (integrin beta-2, ITGB2) is a type I transmembrane glycoprotein that pairs with multiple α subunits (CD11a, CD11b, CD11c, CD11d) to form heterodimeric integrins: LFA-1 (αLβ2), Mac-1 (αMβ2), p150,95 (αXβ2), and αDβ2 (αDβ2).
• These integrins mediate leukocyte adhesion and transmigration by binding ICAMs (1-3), VCAM1, complement iC3b, fibrinogen, and factor X, critical for immune cell trafficking and effector functions (PubMed:1676048, PubMed:23775590, PubMed:38195629, PubMed:29100055, PubMed:10438935, PubMed:8777714, PubMed:9841932, PubMed:11812992, PubMed:28807980, PubMed:18587400). Related references: PMID:1676048, PMID:23775590, PMID:38195629, PMID:29100055, PMID:10438935, PMID:8777714, PMID:9841932, PMID:11812992, PMID:28807980, PMID:18587400
• As a key receptor for apoptotic cell clearance, LFA-1 (αLβ2) cooperates with ICAM3 to promote phagocytosis of apoptotic neutrophils by macrophages (PubMed:23775590). Additionally, αXβ2 recognizes erythrocyte ICAM4 and neuronal ICAM5, linking integrins to erythrophagocytosis and neuron–leukocyte interactions (PubMed:16985175, PubMed:10741396). Related references: PMID:23775590, PMID:16985175, PMID:10741396
• CD18 integrins contribute to natural killer cell cytotoxicity (PubMed:15356110). Mac-1 (αMβ2) is required for CD177‑PRTN3-mediated activation of TNF‑primed neutrophils (PubMed:21193407). Related references: PMID:15356110, PMID:21193407
• Subcellularly, CD18 localizes to the cell membrane and membrane rafts of leukocytes, particularly neutrophils (PubMed:23775590, PubMed:21193407, PubMed:28807980). Related references: PMID:23775590, PMID:21193407, PMID:28807980
• The protein is heavily glycosylated, contains EGF‑like domains, and harbors calcium/magnesium-binding motifs essential for integrin activation. It is post‑translationally modified by phosphorylation and features a pyrrolidone carboxylic acid at its N‑terminus.
• Consistent with its essential role in leukocyte adhesion, ITGB2 mutations are linked to immunodeficiencies such as leukocyte adhesion deficiency (LAD) type I.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminus (aa 1–100) of human CD18; verify reactivity in the intended species/application via positive control lysates (e.g., human, mouse, or rat leukocytes).
• For Western blot (WB), a predominant band at ~85 kDa is expected; consider using reducing conditions and probing with appropriate secondary antibodies.
• For paraffin immunofluorescence (IF‑P) and immunohistochemistry (IHC‑P), optimize antigen retrieval (heat‑induced epitope retrieval is commonly required) and titrate the primary antibody to reduce background.
• ELISA applications may require coating with the recombinant immunogen or native integrin complexes; pair with a detection secondary to establish a standard curve.
• Due to cross‑reactivity with mouse and rat, validate antibody specificity in the relevant model systems when switching between species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material solutions—from validated antibody pairs and recombinant proteins to bulk ancillary reagents—empowering diagnostic manufacturers and research laboratories worldwide. This anti-CD18 monoclonal antibody supports critical immunological studies, and our team can assist with customized sourcing for rare targets, optimized buffer formulations, and technical consultation to streamline your assay development from concept to clinic.