Anti-CD177 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q8N6Q3

Core Product Specifications and Parameters

Biological Background: CD177 Function and Localization

• CD177 is a GPI-anchored glycoprotein expressed on a subset of human neutrophils, also known as NB1 glycoprotein or PRV-1.
• In association with the beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, CD177 mediates activation of TNF-primed neutrophils, including degranulation and superoxide production. Related references: PMID:21193407
• By preventing beta-2 integrin internalization and attenuating chemokine signaling, CD177 favors neutrophil adhesion over migration. Related references: PMID:28807980
• Heterophilic interaction with PECAM1 on endothelial cells contributes to neutrophil transendothelial migration in vitro. Related references: PMID:17580308
• CD177 acts as a receptor for mature proteinase 3 (PRTN3), enabling its display on the neutrophil surface and potentially enhancing vascular integrity during diapedesis. Related references: PMID:17244676, PMID:18462208, PMID:23202369
• Although involved in adhesion and migration processes in vitro, CD177 appears dispensable for neutrophil recruitment during bacterial infection in vivo. Related references: PMID:23461681
• Subcellularly, CD177 localizes to the cell membrane, membrane rafts, cytoplasmic granule membranes, and lamellipodia, and can be secreted.
• Highly expressed in normal bone marrow and weakly in fetal liver; expression during neutrophil differentiation begins at the metamyelocyte stage; the percentage of CD177-positive mature neutrophils varies across individuals and is elevated in polycythemia vera and essential thrombocythemia.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 22-407) of human CD177, supporting detection of the mature, surface-expressed protein.
• For Western blot (WB), consider that CD177 has a predicted molecular weight of ~46 kDa (unprocessed) but typically migrates at ~51 kDa due to glycosylation; validate with appropriate positive controls such as neutrophil lysates.
• For immunohistochemistry (IHC-P), antigen retrieval conditions may need optimization; because CD177 is expressed on only a subset of neutrophils, staining may be heterogeneous.
• In ELISA applications, this antibody may be useful as a capture or detection reagent; pairing with a compatible secondary antibody is advised.
• Cross-reactivity is reported as human; performance in other species should be experimentally validated if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research laboratories, and clinical assay developers streamlined access to premium IVD raw materials, including this CD177 monoclonal antibody. Our portfolio spans validated antibody pairs, recombinant proteins, and ancillary reagents tailored for inflammation, hematology, and autoimmune disease research. From early-stage assay development to clinical validation, CamelBio’s technical expertise and end-to-end support help you move from concept to clinic efficiently.