Anti-CD177 Polyclonal Antibody for WB, ELISA - Q8N6Q3

Core Product Specifications and Parameters

Biological Background: CD177 Function and Localization

• CD177 mediates activation of TNF-primed neutrophils, including degranulation and superoxide production, in association with beta-2 integrin ITGAM/CD11b and ITGB2/CD18. Related references: PMID:21193407
• By preventing beta-2 integrin internalization and attenuating chemokine signaling, CD177 favors neutrophil adhesion over migration. Related references: PMID:28807980
• CD177 heterophilic interaction with PECAM1 on endothelial cells facilitates neutrophil transendothelial migration in vitro, though it appears dispensable for in vivo neutrophil recruitment during bacterial infection. Related references: PMID:17580308, PMID:23461681
• Acts as a receptor for mature protease PRTN3, enabling its display on the neutrophil cell surface. Related references: PMID:17244676, PMID:18462208
• By displaying PRTN3, CD177 may enhance endothelial junctional integrity and vascular integrity during neutrophil diapedesis. Related references: PMID:23202369
• Subcellularly, CD177 localizes to the cell membrane, membrane rafts, secreted forms, cytoplasmic granule membranes, and lamellipodia.
• Highly expressed in normal bone marrow; during neutrophil differentiation, expression begins at the metamyelocyte stage. Expressed only in a subset of mature neutrophils, with percentage varying across individuals. Also upregulated in polycythemia vera and essential thrombocythemia granulocytes.
• Keywords: Alternative splicing, Cell adhesion, GPI-anchor, Glycoprotein, Immunity, Innate immunity, Lipoprotein, Membrane, Repeat, Secreted, Signal.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 35–285 of human CD177, encompassing the extracellular domain likely recognized in native and denatured forms. For WB, consider a reducing or non-reducing protocol as the protein contains disulfide bonds; the predicted molecular weight is 51 kDa, but glycosylation may shift the apparent band.
• For ELISA applications, consider pairing with a validated detection antibody reactive to a non-overlapping epitope, and include appropriate antigen controls.
• CD177 expression is restricted to a subset of neutrophils; validate reactivity using primary human neutrophils or suitable cell lines that endogenously or ectopically express CD177.
• The antibody cross-reacts with human and mouse CD177, enabling cross-species studies; perform internal validation for each species and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. This CD177 polyclonal antibody supports assay development in neutrophil biology and inflammation, and we can further supply validated antibody pairs, bulk ancillary reagents, and custom rare-target raw material sourcing to accelerate your pipeline from concept to clinic.