Anti-CD172a/SIRPα Polyclonal Antibody for WB, IF/ICC, ELISA - P78324

Core Product Specifications and Parameters

Biological Background: CD172a/SIRPα Function and Localization

• SIRPα (also known as SHPS-1, CD172a, p84) is a tyrosine-protein phosphatase non-receptor type substrate 1 encoded by the SIRPA gene in humans.
• Functions as an immunoglobulin-like cell surface receptor for CD47, acting as a docking protein that recruits PTPN6 and PTPN11 to the plasma membrane upon activation.
• Negatively regulates receptor tyrosine kinase-coupled signaling triggered by cell adhesion, growth factors, or insulin, and mediates inhibition of phagocytosis, mast cell activation, and dendritic cell maturation.
• Binding to CD47 on T-cells and other cells prevents maturation of immature dendritic cells and suppresses cytokine production by mature dendritic cells.
• Serves as a receptor for thrombospondin-1 (THBS1), which stimulates SIRPα phosphorylation and triggers NADPH oxidase-derived ROS production in non-phagocytic cells. Related references: PMID:24511121
• Plays a role in antiviral immunity by limiting new world arenavirus infection through decreased virus internalization.
• Highly expressed in brain and detected on myeloid cells, but not T-cells; also found at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle, and pancreas.
• Post-translationally modified: contains disulfide bonds, glycosylation, and phosphorylation; belongs to the immunoglobulin superfamily with three Ig-like domains and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 220-375 within the cytoplasmic domain, making it suitable for detecting total SIRPα protein by Western blotting (WB).
• For immunofluorescence/immunocytochemistry (IF/ICC), consider permeabilization protocols to allow antibody access to the intracellular epitope.
• Validate antibody specificity using control cell lines with known SIRPα expression levels (e.g., brain tissue or myeloid cells) and appropriate negative controls.
• Cross-reactivity with mouse and rat SIRPα has been reported; however, individual experimental conditions may vary; confirm performance in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing research-grade and bulk quantities of antibodies, antigens, and ancillary reagents. This anti-CD172a/SIRPα polyclonal antibody supports studies in immune checkpoint biology, phagocytosis regulation, and neurodevelopmental signaling. Beyond individual products, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, and custom sourcing for rare targets to accelerate your diagnostic development from concept to clinic.