Anti-CD171/L1CAM Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P32004

Core Product Specifications and Parameters

Biological Background: CD171/L1CAM Function and Localization

• Neural cell adhesion molecule L1 (L1CAM, also designated CD171) is a cell adhesion molecule of the immunoglobulin superfamily that exists in multiple isoforms via alternative splicing.
• It mediates cell adhesion dynamics and generates transmembrane signals at tyrosine kinase receptors, playing essential roles in nervous system development and function.
• During brain development, L1CAM is critical for neuronal migration, axonal growth and fasciculation, and synaptogenesis; in the adult brain, it contributes to synaptic plasticity and structural dynamics.
• L1CAM localizes to the plasma membrane and is enriched at specialized neuronal projections, including growth cones, axons, and dendrites.
• The protein is a glycophosphoprotein with immunoglobulin-like domains and disulfide bonds, and its phosphorylation regulates adhesive and signaling functions.
• Mutations in L1CAM are associated with a spectrum of neurological disorders, including X-linked hydrocephalus, MASA syndrome, spastic paraplegia type 1, and agenesis of the corpus callosum.
• It is predominantly expressed in the nervous system and has been implicated in cell migration and differentiation beyond neuronal contexts.

Experimental Guidance and Technical Tips

• Immunogen design: The immunogen targets the intracellular C-terminal region (amino acids 1143-1257) of L1CAM, conserved across human, mouse, and rat, supporting the observed cross-reactivity. This region is not expected to be glycosylated, so the antibody may recognize both glycosylated and unglycosylated forms.
• Western blotting: The predicted molecular weight is 140 kDa, but extensive glycosylation typically shifts the apparent band to ~200-220 kDa. Use reducing and denaturing conditions, and validate with brain or neuronal cell lysates.
• Immunofluorescence/ICC: Permeabilization is required to detect the intracellular epitope. Fixation with 4% PFA and permeabilization with 0.1% Triton X-100 are suggested. Co-stain with neuronal markers to confirm specificity in neurons and projections.
• ELISA: The antibody can be used as a detection reagent in direct or indirect ELISA formats. For sandwich ELISA, pair with a capture antibody targeting a distinct epitope. Titration and optimization are essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials. For neuroscience and cell adhesion targets like CD171/L1CAM, we offer validated polyclonal antibodies, matched antibody pairs, and bulk ancillary reagents to build robust immunoassays. Our one-stop platform also supports rare target sourcing and technical consultation, accelerating your transition from concept to clinic.