Anti-CD171/L1CAM Monoclonal Antibody for WB, IHC-P, ELISA - P32004

Core Product Specifications and Parameters

Biological Background: CD171/L1CAM Function and Localization

• Encoded by the L1CAM gene (also known as CAML1 and MIC5), L1CAM is a member of the immunoglobulin superfamily of cell adhesion molecules.
• During brain development, L1CAM is critical for neuronal migration, axonal growth, fasciculation, and synaptogenesis.
• In the mature brain, it contributes to the dynamics of neuronal structure and function, including synaptic plasticity.
• Mediates cell adhesion and generates transmembrane signals at tyrosine kinase receptors.
• Expressed on the cell membrane and enriched in cell projections such as growth cones, axons, and dendrites.
• Post-translational modifications include phosphorylation, glycosylation, and disulfide bond formation.
• Mutations in L1CAM are associated with neurological disorders, including hereditary spastic paraplegia (SPG1), Hirschsprung disease, and X-linked hydrocephalus.
• The canonical protein has a molecular weight of approximately 140 kDa, with alternative splicing producing multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans the entire extracellular domain (amino acids 20-1120), offering broad recognition of native L1CAM.
• For Western blot, a protein band near 140 kDa is expected; low-percentage gels and longer transfer times are recommended for high-molecular-weight proteins.
• In paraffin-embedded immunohistochemistry (IHC-P), heat-induced epitope retrieval (HIER) may enhance staining; optimize primary antibody dilution and detection system for your tissue type.
• The antibody is suitable for ELISA and can be paired with appropriate secondary reagents; validate matrix effects in your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For neuroscience and cell adhesion research, we offer validated anti-L1CAM monoclonal antibodies, optimized for applications like Western blot and immunohistochemistry. Our supply capabilities include validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development and commercialization.