Anti-CD170/Siglec-5 Monoclonal Antibody for Flow Cytometry - O15389

Core Product Specifications and Parameters

Biological Background: Siglec-5 (CD170) Function and Localization

• Sialic acid-binding immunoglobulin-like lectin 5 (Siglec-5), also known as CD170, CD33L2, or OBBP2, is a member of the Siglec family of transmembrane receptors that recognize sialic acid-containing glycans.
• It functions as a putative adhesion molecule that mediates sialic acid-dependent binding to cells, with equal affinity for α-2,3-linked and α-2,6-linked sialic acids.
• The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface, modulating its binding activity.
• Siglec-5 is a membrane protein with an immunoglobulin-like domain and is predominantly expressed on monocytic and myeloid lineage cells, including peripheral blood monocytes and neutrophils.
• Tissue expression is high in peripheral blood leukocytes, spleen, and bone marrow, and lower in lymph node, lung, appendix, placenta, pancreas, and thymus.
• It contains disulfide bonds and is classified as a lectin and cell adhesion molecule, with structural features including Ig domains and a transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant fusion protein spanning amino acids 17-434 (the extracellular domain) of human Siglec-5; this design captures the complete sialic acid-binding region, making it suitable for detecting native CD170 on live cells by flow cytometry (FC).
• Since Siglec-5 is expressed on monocytes and neutrophils, consider using peripheral blood mononuclear cells (PBMCs) or whole blood as sample systems for FC; validate the antibody’s reactivity in your specific experimental setup.
• For FC, use standard staining protocols with appropriate isotype controls; note that the antibody is labeled with dye, which requires compatible laser and filter configurations.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We offer validated antibody pairs, optimized monoclonal antibodies like this anti-CD170 clone, bulk ancillary reagents, and rare target raw-material sourcing to support your myeloid marker and immune cell profiling assays. Let us be your sourcing bridge for high-quality raw materials, accelerating your IVD development from research to commercialization.