Anti-CD168/RHAMM Rabbit Polyclonal Antibody (KO Validated) for WB, IF, ELISA - O75330

Core Product Specifications and Parameters

Biological Background: CD168/RHAMM Function and Localization

• CD168/RHAMM (HMMR) is a receptor for hyaluronic acid (HA) that mediates HA-dependent cell motility and focal adhesion kinase (PTK2/FAK1) phosphorylation.
• The protein contributes to cellular transformation, metastasis formation, and regulation of extracellular-regulated kinase (ERK) signaling.
• HMMR undergoes alternative splicing and contains repetitive sequence motifs; it is classified as a glycoprotein and phosphoprotein.
• Localized at the cell surface, within the cytoplasm, and associates with the mitotic spindle, implicating roles in cell division and motility.
• Expressed in testis and breast tissue, with potential involvement in reproductive biology and breast cancer.
  Related references: PMID:22965910 PMID:8890751
• The predicted molecular weight is approximately 84 kDa, consistent with the observed protein weight.
• Hyaluronan binding may trigger signaling pathways involved in adipogenesis and cellular differentiation (By similarity).

Experimental Guidance and Technical Tips

• This antibody targets the N-terminal region (amino acids 1–300) of human CD168/RHAMM; assess epitope accessibility if using truncated or modified proteins.
• For Western blotting, anticipate a band near 84 kDa; validate with positive control lysates from testis or breast cancer cell lines expressing CD168.
• In immunofluorescence, expect cytoplasmic and spindle-associated staining; co-stain with a mitotic marker (e.g., phospho-histone H3) to confirm localization.
• The antibody has been knockout (KO) validated by the supplier; confirm specificity in your system using KO or knockdown controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with end-to-end access to IVD raw materials. This anti-CD168/RHAMM polyclonal antibody supports investigations into cancer metastasis and cell motility, and CamelBio's sourcing network can supply additional validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials to accelerate your assay development.