Anti-CD168/RHAMM Polyclonal Antibody for WB, ELISA - O75330

Core Product Specifications and Parameters

Biological Background: CD168/RHAMM Function and Localization

• Encoded by the HMMR gene, CD168/RHAMM (Hyaluronan Mediated Motility Receptor) is also known as Intracellular Hyaluronic Acid-Binding Protein (IHABP) and Receptor for Hyaluronan-Mediated Motility (RHAMM). The protein undergoes alternative splicing.
• Functions as a receptor for hyaluronic acid (HA), playing a key role in cell motility. Upon HA binding, it triggers phosphorylation of multiple proteins, including PTK2/FAK1, and regulates extracellular-regulated kinase (ERK) activity.
• Implicated in cellular transformation and metastasis formation, and may also act as a regulator of adipogenesis.
• Exhibits a dual subcellular distribution, localizing to the cell surface, cytoplasm, and the cytoskeleton, specifically associated with the spindle apparatus.
• Expressed in testis and breast tissue. Related references: PMID:22965910 PMID:8890751
• Classified under keywords such as glycoprotein, phosphoprotein, and hyaluronic acid-binding, indicating post-translational modifications and functional interactions.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 341-440 of human CD168/RHAMM (isoform based on NP_001136028.1). For detection of other isoforms or species variants, consider verifying epitope conservation.
• This polyclonal antibody is suitable for western blot (WB) and ELISA. The predicted molecular weight is 84 kDa; in WB, validate band size in relevant cell or tissue lysates, considering possible post-translational modifications.
• Cross-reactivity has been confirmed for human, mouse, and rat samples. For other species, perform sequence alignment or pilot testing to assess suitability.
• Since CD168/RHAMM is expressed in breast and testis, these tissues may serve as positive controls. Adjust sample preparation to account for its association with the cytoskeleton and spindle apparatus.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers, laboratories, and research institutes, offering comprehensive access to IVD raw materials, technical services, and consulting from concept to clinic. This anti-CD168/RHAMM antibody supports research in cell motility, metastasis, and hyaluronan signaling. Beyond single antibodies, CamelBio provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your assay development.