Anti-CD168/RHAMM Monoclonal Antibody for WB, IHC-P, ELISA - O75330

Core Product Specifications and Parameters

Biological Background: CD168/RHAMM Function and Localization

• CD168/RHAMM (HMMR) is a receptor for hyaluronic acid (HA) with a predicted molecular weight of ~84 kDa; multiple isoforms arise from alternative splicing.
• Upon HA binding, HMMR triggers phosphorylation of downstream proteins, including PTK2/FAK1, thereby regulating cell motility and adhesion (By similarity).
• HMMR is implicated in cellular transformation, metastasis formation, and modulation of extracellular signal-regulated kinase (ERK) activity.
• It may also function as a regulator of adipogenesis (By similarity).
• Subcellularly, HMMR localizes to the cell surface, cytoplasm, and associates with the cytoskeleton and spindle apparatus.
• Expression is documented in testis (PubMed:22965910) and breast (PubMed:8890751). Related references: PMID:22965910, PMID:8890751
• Key post‑translational modifications include phosphorylation and glycosylation (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (aa 1–100); the antibody may recognize full‑length CD168/RHAMM and isoforms containing this epitope but could miss splice variants lacking it.
• For Western blot, a band near 84 kDa is predicted; additional bands from alternative splicing or post‑translational modifications may be observed.
• In immunohistochemistry (paraffin‑embedded sections), consider antigen retrieval and note expected expression in tissues such as testis and breast.
• For ELISA, the peptide immunogen can serve as a positive control; validate detection of the native protein in relevant biological matrices.
• Cross‑reactivity with human and mouse is stated; verify performance in your sample system if working with other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized recombinant antibodies like this CD168/RHAMM rabbit mAb, supporting research into hyaluronan‑driven cell motility and oncology applications. Our integrated sourcing platform delivers bulk ancillary reagents and rare‑target raw materials, streamlining immunoassay development from concept to clinic. Partner with CamelBio for reliable supply and technical continuity across your diagnostic pipeline.