Anti-CD166/ALCAM Monoclonal Antibody for WB, IF/ICC, ELISA - Q13740

Core Product Specifications and Parameters

Biological Background: CD166/ALCAM Function and Localization

• CD166/ALCAM, also known as activated leukocyte cell adhesion molecule (ALCAM) or MEMD, is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. It contains five extracellular Ig-like domains, a single transmembrane helix, and a short cytoplasmic tail.
• CD166/ALCAM mediates both heterotypic cell-cell contacts via interaction with CD6 and homotypic interactions with ALCAM-expressing cells, playing critical roles in T-cell activation and proliferation. Related references: PMID:15048703, PMID:16352806, PMID:24945728
• It contributes to the formation and maturation of the immunological synapse through its interaction with CD6, enhancing immune responses. Related references: PMID:15294938, PMID:16352806
• Acts as a ligand for the inhibitory receptor LILRB4, thereby enhancing LILRB4-mediated inhibition of T-cell proliferation. Related references: PMID:29263213
• Required for normal hematopoietic stem cell engraftment in the bone marrow and plays a role in hematopoiesis; required for maintenance of hematopoietic stem cell numbers. Related references: PMID:24740813
• Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction, and inhibits endothelial cell migration while promoting endothelial tube formation. Related references: PMID:23169771, PMID:15496415
• Promotes neurite extension, axon growth, and axon guidance; retinal ganglion cell axons grow preferentially on ALCAM-coated surfaces.
• Subcellular localization: primarily expressed on the cell membrane, but also found on cell projections like axons and dendrites; a secreted isoform exists. Detected on hematopoietic stem cells, lymphocytes, monocytes, dendritic cells, endothelial cells, and various tissues including spleen, placenta, and liver. Related references: PMID:24740813, PMID:23169771, PMID:15048703, PMID:16352806, PMID:9502422, PMID:7760007, PMID:15496415

Experimental Guidance and Technical Tips

• The immunogen corresponds to the entire extracellular domain (amino acids 28-526), ensuring recognition of the native protein. This makes the antibody suitable for detecting CD166/ALCAM in its native conformation in applications like IF/ICC and ELISA.
• For Western blotting (WB), a band around 65 kDa is expected; consider using appropriate loading controls and optimize protein extraction methods for membrane proteins.
• In IF/ICC, anticipate membrane and possibly punctate staining along cellular projections; validate with positive and negative cell lines (e.g., T-cells, melanoma lines).
• For ELISA, pair with a suitable secondary antibody and validate assay conditions using recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies. Targeting CD166/ALCAM, critical in immunology and oncology research, we offer this rabbit monoclonal antibody as part of our extensive catalog to support your diagnostic development and basic research. Beyond antibodies, we source bulk ancillary reagents and rare target raw materials, streamlining your procurement from concept to clinic.