Anti-CD166/ALCAM Monoclonal Antibody () for Flow Cytometry - Q13740

Core Product Specifications and Parameters

Biological Background: CD166/ALCAM Function and Localization

• CD166 antigen (ALCAM, activated leukocyte cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin superfamily. Alternative splicing yields membrane-bound and secreted isoforms.
• CD166/ALCAM binds the T-cell surface receptor CD6, enhancing T-cell activation and proliferation. Related references: PMID:15048703, PMID:16352806, PMID:24945728
• Homophilic ALCAM-ALCAM interactions contribute to immunological synapse maturation and dendritic cell–endothelial adhesion. Related references: PMID:15294938, PMID:15496415, PMID:16352806, PMID:23169771
• ALCAM serves as a ligand for the inhibitory receptor LILRB4, potentiating LILRB4-mediated suppression of T-cell proliferation. Related references: PMID:29263213
• ALCAM is required for the engraftment and maintenance of hematopoietic stem cells in the bone marrow. Related references: PMID:24740813
• In endothelial cells, homotypic ALCAM interactions inhibit migration and promote tube formation, and the protein is essential for normal lymphatic vessel organization. Related references: PMID:15496415, PMID:23169771
• A secreted splice variant competes with membrane-bound isoforms for interaction partners, inhibiting cell attachment and promoting endothelial migration.
• CD166/ALCAM localizes to the cell membrane, cell projections (axons, dendrites), and is also secreted. It is expressed on activated T-cells, B-cells, monocytes, endothelial cells, and several tissues including spleen, placenta, and liver.

Experimental Guidance and Technical Tips

• The antibody is supplied as an conjugate, ready for direct flow cytometry; no secondary detection reagent is needed.
• Perform a titration (e.g., 0.1–5 µg per million cells) to determine the optimal staining concentration for your specific cellular system.
• Human peripheral blood mononuclear cells (PBMCs) or CD166/ALCAM-expressing cell lines (such as activated T-cells) can serve as positive controls; include an appropriate isotype control.
• Protect the antibody from light and store at 2–8°C; do not freeze.
• For multicolor panels, verify fluorochrome compatibility with (excitation/emission: 488/520 nm).
• The immunogen spans amino acids 28–526, covering the majority of the extracellular domain; consider validation for detection of both membrane-bound and soluble forms in your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material procurement, from research-grade antibodies to bulk custom production. This anti-CD166/ALCAM monoclonal antibody supports flow cytometry applications in immune cell profiling and cancer biology. We also offer validated antibody pairs, blocking buffers, and ancillary reagents to streamline your assay development workflow.