Anti-CD163 Rabbit PolymAb® for WB, IF-P, IHC-P, ELISA - Q86VB7 / Q2VLH6

Core Product Specifications and Parameters

Biological Background: CD163 Function and Localization

• CD163, also known as M130 or hemoglobin scavenger receptor, is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. It functions as an acute phase-regulated receptor on macrophages.
• It mediates clearance and endocytosis of hemoglobin/haptoglobin complexes, protecting tissues from oxidative damage and facilitating iron recycling.
• Binds hemoglobin/haptoglobin in a calcium- and pH-dependent manner, with higher affinity for multimeric haptoglobin phenotypes.
• Upon ligand binding, CD163 triggers intracellular signals involving tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and secretion of IL6 and CSF1.
• The protein exists as membrane-bound and secreted (soluble sCD163) forms; sCD163 may have anti-inflammatory roles and serve as a biomarker for macrophage activation.
• Subcellular localization includes the cell membrane and secreted. CD163 is predominantly expressed in tissue-resident macrophages (e.g., Kupffer cells, splenic red pulp macrophages) and monocytes, with isoform 3 being the major blood isoform.
• Post-translational modifications include glycosylation and phosphorylation; keywords: alternative splicing, acute phase, disulfide bond, glycoprotein, inflammatory response, phosphoprotein, repeat, signal, transmembrane.
• Molecular weight is approximately 125 kDa (UniProt), consistent with detected bands around 121–125 kDa depending on glycosylation and species.

Experimental Guidance and Technical Tips

• The immunogen is recombinant protein covering human and mouse CD163; the antibody may recognize conserved epitopes—validate in your specific sample system.
• For Western blot, CD163 runs at approximately 121–125 kDa; glycosylation may cause band shifts. Use reducing conditions and ensure adequate transfer of high-molecular-weight proteins.
• For immunohistochemistry (IHC-P), antigen retrieval (e.g., citrate buffer, heat-induced) may be required; positive control tissues include liver, spleen, or inflamed tissues.
• In immunofluorescence (IF-P), optimize permeabilization and blocking steps; CD163 localizes to the cell membrane and intracellular compartments.
• For ELISA, the antibody may be suitable for capture or detection of soluble CD163; validate pair compatibility if used with other antibodies.
• Cross-reactivity with rat and cynomolgus monkey suggests conserved epitopes, but always confirm reactivity in your target species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with premium raw materials, including this anti-CD163 PolymAb® for macrophage and inflammation studies. Beyond individual antibodies, we offer validated antibody pairs, bulk reagents, and custom sourcing for rare targets. Contact us to streamline your assay development with reliable, one-stop procurement solutions.