Anti-CD163 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - Q86VB7

Core Product Specifications and Parameters

Biological Background: CD163 (M130) Function and Localization

• CD163 (also known as Scavenger receptor cysteine-rich type 1 protein M130, M130, MM130, SCARI1) is an acute phase-regulated receptor primarily involved in the clearance of hemoglobin/haptoglobin complexes by macrophages, protecting tissues from oxidative damage.
• It binds hemoglobin/haptoglobin complexes in a calcium- and pH-dependent manner, triggering endocytosis and subsequent breakdown of heme for iron recycling, while also inducing intracellular signaling cascades that promote cytokine secretion (e.g., IL6, CSF1).
• Isoform 3 (the predominant blood isoform) exhibits higher endocytic capacity and surface expression compared to other isoforms.
• The soluble form (sCD163), generated by shedding, functions as an anti-inflammatory mediator and serves as a diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
• CD163 is localized both on the cell membrane of monocytes and mature tissue macrophages (e.g., Kupffer cells, splenic red pulp macrophages) and in a secreted form.
• It contains a signal peptide, multiple scavenger receptor cysteine-rich (SRCR) domains, a transmembrane region, and is subject to post-translational modifications including glycosylation, phosphorylation, and disulfide bond formation.
• Expressed highly in liver Kupffer cells, splenic red pulp macrophages, cortical thymic macrophages, bone marrow resident macrophages, and meningeal macrophages.

Experimental Guidance and Technical Tips

• For Western blot (WB), note the predicted molecular weight of ~125 kDa; adjust gel percentage and transfer time accordingly to ensure efficient blotting of high-molecular-weight proteins.
• In immunofluorescence on paraffin sections (IF-P), antigen retrieval conditions may impact epitope accessibility; optimize heat-induced epitope retrieval (HIER) or enzymatic methods for your tissue type.
• The immunogen is a synthetic peptide spanning the cytoplasmic domain (aa 861–961), which may perform well in total protein detection; however, for sandwich ELISA, test pairing compatibility with antibodies targeting distinct epitopes.
• As cross-reactivity is restricted to human, validate assay specificity using appropriate human cell lines or tissue samples, and consider isotype controls for multiplexed experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-CD163 antibody is well-suited for studies on macrophage biology, inflammation, and iron metabolism, and can be integrated into custom assay development workflows. With a focus on seamless procurement and technical support, CamelBio streamlines the transition from concept to clinic.