Anti-CD162/PSGL-1 Rabbit PolymAb® Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - Q14242 / Q3TA56

Core Product Specifications and Parameters

Biological Background: CD162/PSGL-1 Function and Localization

• CD162, also known as P-selectin glycoprotein ligand 1 (PSGL-1), is a SLe(x)-type proteoglycan encoded by the SELPLG gene. It exists as alternative splicing isoforms.
• It mediates leukocyte rolling on vascular surfaces through high-affinity, calcium-dependent interactions with E-, P-, and L-selectins, playing a critical role in the initial steps of inflammation.
• The protein is expressed on the surface of neutrophils, monocytes, and most lymphocytes, localizing to the cell membrane.
• PSGL-1 functions as a host cell receptor for enterovirus 71, facilitating viral entry.
• Extensive post-translational modifications, including sialic acid, sulfation, and glycosylation, are essential for its selectin-binding activity.
• The CD162/PSGL-1 protein is a transmembrane protein with a calculated molecular weight of approximately 43 kDa, featuring disulfide bonds and a signal peptide.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was generated using a recombinant protein immunogen, which may ensure recognition of native epitopes; confirm performance in your specific sample system.
• For Western blot analysis, consider the predicted molecular weight of ~43 kDa and optimize loading controls.
• In immunohistochemistry (IHC) and immunofluorescence (IF/ICC, IF-P), validate the antibody on appropriate positive and negative tissue/cell samples.
• For ELISA, titrate the antibody and include appropriate controls to assess specificity and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to validated IVD raw materials, including this CD162/PSGL-1 antibody, ideal for inflammation and host-pathogen interaction studies. From optimized monoclonal antibodies to bulk ancillary reagents, we support your entire development pipeline with one-stop sourcing and expert technical consultation.