Anti-CD162/PSGL-1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14242

Core Product Specifications and Parameters

Biological Background: CD162/PSGL-1 Function and Localization

• P-selectin glycoprotein ligand 1 (PSGL-1), also known as CD162, is encoded by the SELPLG gene.
• Functions as a SLe(x)-type proteoglycan that mediates leukocyte rolling on vascular surfaces through high-affinity, calcium-dependent interactions with E-, P-, and L-selectins, critical for the initial steps of inflammation.
• Acts as a receptor for enterovirus 71, facilitating viral entry.
• Localized to the plasma membrane as a type I transmembrane protein.
• Expressed on neutrophils, monocytes, and most lymphocytes.
• Undergoes post-translational modifications including sulfation, sialylation, phosphorylation, disulfide bond formation, and contains pyrrolidone carboxylic acid.
• Subject to alternative splicing and classified under host cell receptor for virus entry membrane proteins.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 42-295), enabling recognition of the native protein on cell surfaces.
• For Western blot, note that glycosylation may cause the apparent molecular weight to be higher than the predicted 43-44 kDa; consider deglycosylation for precise detection.
• Validate antibody reactivity in appropriate cell lines or tissues known to express PSGL-1, such as HL-60 or human leukocytes.
• For immunohistochemistry, optimize antigen retrieval and test a dilution range to minimize background.
• In immunofluorescence, a membrane staining pattern is expected; permeabilization may be omitted for surface detection.
• This antibody can be employed in ELISA for quantitative detection; pair with a compatible secondary antibody or use in a sandwich format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents for inflammation and cell adhesion research. Whether you need optimized CD162/PSGL-1 antibodies or related selectin ligands, we streamline sourcing from concept to clinic.