Anti-CD162/PSGL-1 Rabbit Monoclonal Antibody for IF-P, IHC-P, FC, ELISA - Q3TA56

Core Product Specifications and Parameters

Biological Background: CD162/PSGL-1 Function and Localization

• CD162/PSGL-1 is encoded by the Selplg gene in mouse, with alternative names including Psgl1, Selp1, and Selpl.
• The UniProt entry Q3TA56 is currently unreviewed (TrEMBL) and predicts a molecular weight of 43852 Da, consistent with the observed band at 44 kDa.
• Keywords assigned to this entry include membrane, signal, and transmembrane helix, indicating localization to the plasma membrane.
• The presence of a signal peptide and a transmembrane domain supports its classification as a type I transmembrane protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 18-320), making this antibody suitable for detecting surface-expressed CD162/PSGL-1 in applications like flow cytometry (FC) and immunohistochemistry (IHC-P).
• For immunofluorescence (IF-P), consider permeabilization protocols; the antibody may detect both surface and intracellular protein pools.
• ELISA performance may be optimized using recombinant protein or cell lysates as the coating antigen.
• Cross-reactivity with rat is reported; always validate specificity in your target species and tissue system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your procurement of IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For CD162/PSGL-1-related projects, we support assay development with curated reagents and consultative supply solutions, bridging the gap from concept to clinical application.