Anti-CD162/PSGL-1 Monoclonal Antibody for WB, IF/ICC, FC, ELISA - Q14242

Core Product Specifications and Parameters

Biological Background: CD162/PSGL-1 Function and Localization

• CD162 (PSGL-1) is a SLe(x)-type proteoglycan that serves as a ligand for E-, P-, and L-selectins, mediating leukocyte rolling on vascular endothelium during inflammation.
• It plays a critical role in initial leukocyte capture and recruitment to sites of inflammation.
• The protein is a disulfide-linked homodimer and undergoes extensive post-translational modifications, including sialylation, sulfation, and phosphorylation, which are essential for selectin binding.
• CD162 is expressed on the surface of neutrophils, monocytes, and most lymphocytes.
• It is a transmembrane protein localized to the plasma membrane, with a signal peptide and a tandem repeat region.
• Alternative splicing generates multiple isoforms of SELPLG, the gene encoding CD162.
• Additionally, CD162 functions as a receptor for enterovirus 71 during microbial infection.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 42–295 of the extracellular domain; for Western blot analysis, consider using reducing and non-reducing conditions to evaluate the disulfide-linked dimeric forms.
• In immunofluorescence and flow cytometry applications, the antibody can detect surface-localized CD162 on live cells without permeabilization.
• For ELISA, the antibody may be used as a capture or detection reagent; optimal dilution and pairing should be validated in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports researchers and diagnostic developers working on inflammation, cell adhesion, and leukocyte biology with a comprehensive portfolio of IVD raw materials. For CD162/PSGL-1 and related selectin pathway targets, we can source validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare-target raw materials to streamline your assay development and manufacturing workflows.