Anti-CD161 Rabbit Monoclonal Antibody for WB, IF-P, FC, ELISA - Q12918

Core Product Specifications and Parameters

Biological Background: CD161 Function and Localization

• CD161, encoded by the KLRB1 gene, is a killer cell lectin-like receptor also known as NKR-P1A, CLEC5B, or HNKR-P1a.
• It functions as an inhibitory receptor on natural killer (NK) cells; activation stimulates acid sphingomyelinase (SMPD1), elevating intracellular ceramide and leading to downstream AKT1 and RPS6KA1 kinase activation.
• CD161 activation markedly enhances anti-CD3-induced T-cell proliferation, indicating a co-stimulatory role in certain contexts.
• As a C-type lectin, it binds terminal Gal-alpha(1,3)Gal epitopes and N-acetyllactosamine, and also interacts with the ligand CLEC2D/LLT1 to inhibit NK cell-mediated cytotoxicity and interferon-gamma secretion.
• The protein is a membrane receptor with a signal-anchor, a single transmembrane helix, and a C-type lectin domain.
• It is expressed in a subset of NK cells, preferentially in intestinal epithelium and liver, and in peripheral blood T-cells with a memory antigenic phenotype.
• Key structural features include disulfide bonds and N-linked glycosylation, as evidenced by its glycoprotein classification.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 67-225), suggesting the antibody likely targets the ligand-binding region; consider this when designing blocking or competition experiments.
• For Western blot, a band near 25 kDa is expected; use reduced/denatured conditions and validate with positive controls such as NK92 cells or activated PBMCs.
• In flow cytometry, CD161 surface staining on NK and T-cell subsets may require Fc receptor blocking; titrate the antibody to optimize signal-to-noise.
• For IF-P, antigen retrieval may be necessary; validate in tissues known to harbor CD161+ lymphocytes (e.g., liver or intestinal sections).
• Species cross-reactivity with mouse and rat enables preclinical studies, but confirm reactivity in your specific model system.
• Pair this antibody with appropriate isotype controls and validate performance in each application with relevant biological samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive procurement platform for IVD raw materials, providing validated antibodies, recombinant proteins, and supporting reagents to diagnostics manufacturers and research laboratories worldwide. This anti-CD161 monoclonal antibody is suited for immune-oncology and NK cell-targeted projects, and can be integrated into panel development or functional assays. Leveraging deep sourcing networks, CamelBio simplifies supply chains for rare specificities, bulk orders, and ancillary reagents, accelerating your journey from concept to clinic.