Anti-CD16 Monoclonal Antibody for Flow Cytometry - P08637 / O75015

Core Product Specifications and Parameters

Biological Background: CD16 Function and Localization

• CD16, also known as FCGR3A/FCGR3B, is a low-affinity receptor for the Fc fragment of IgG. It binds clustered antigen-IgG complexes on cell surfaces, triggering antibody-dependent cellular cytotoxicity (ADCC) without engaging free monomeric IgG. Related references: PMID:11711607 PMID:21768335 PMID:22023369
• On natural killer (NK) cells, CD16 mediates IgG effector functions. Upon infection, it senses antigen-IgG complexes, inducing cytokine production and degranulation to control viral load. It also drives the generation of adaptive memory NK cells with high IFNG output. Related references: PMID:24412922 PMID:25786175
• CD16 signaling, via ITAM-containing adapters and PKC-mediated phosphorylation, mobilizes intracellular calcium, leading to pro-inflammatory cytokine release or degranulation depending on the signaling context. Related references: PMID:1825220 PMID:23024279 PMID:2532305
• CD16 contributes to NK cell survival and proliferation by preventing apoptosis of NK cell progenitors. Related references: PMID:29967280 PMID:9916693
• Beyond IgG binding, CD16 can costimulate NK cells to lyse target cells independently, enhancing antitumor responses. Related references: PMID:10318937 PMID:23006327
• CD16 is critical for the antitumor activity of therapeutic antibodies; it mediates ADCC of tumor cells and shows enhanced opsonization with afucosylated IgGs. Related references: PMID:27670158 PMID:34485821 PMID:28566370
• In Dengue virus pathogenesis, CD16 participates in antibody-dependent enhancement (ADE) by facilitating viral entry into myeloid cells through afucosylated IgG1 immune complexes.
• CD16 is a transmembrane glycoprotein localized to the cell membrane, with a soluble form also secreted. It is expressed on NK cells and a subset of circulating monocytes.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular region (amino acids 20–208) of both FCGR3A and FCGR3B, encompassing the IgG-binding domains; this design supports detection of native surface CD16 on live cells.
• This antibody is validated for flow cytometry (FC). For optimal staining, consider titrating the antibody on your target cell type and always include appropriate isotype controls.
• Verify cross-reactivity in cynomolgus samples, as sequence homology may affect epitope recognition; pilot studies are recommended when switching species.
• For FC experiments involving NK cells or monocytes, gentle handling and cold staining buffers are advised to minimize receptor shedding or internalization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop shop for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. This anti-CD16 antibody is part of our expanding portfolio of immuno-oncology reagents, suitable for detecting Fc gamma receptors in flow cytometry. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal preparations, bulk ancillary reagents, and custom sourcing for rare targets—helping you streamline procurement and accelerate assay development.