Anti-CD16 Biotinylated Monoclonal Antibody for FC - P08637 / O75015

Core Product Specifications and Parameters

Biological Background: CD16 Function and Localization

• CD16 is the low affinity immunoglobulin gamma Fc region receptor III-A (FCGR3A), also designated CD16a, Fc-gamma RIII-alpha, and FcRIIIa.
• Functions as a receptor for the Fc fragment of IgG, optimally activated by clustered antigen-IgG complexes on cell surfaces to mediate antibody-dependent cellular cytotoxicity (ADCC) without binding free monomeric IgG. Related references: PMID:11711607 PMID:21768335 PMID:22023369
• In NK cells, CD16-mediated signaling involves phosphorylation of ITAM-containing adapters, activation of PI3K, and sustained intracellular calcium elevation, culminating in pro-inflammatory cytokine release and degranulation. Related references: PMID:1825220 PMID:23024279 PMID:2532305
• CD16 plays a key role in NK cell survival, proliferation, and the generation of memory-like adaptive NK cells with enhanced IFN-γ production and anti-viral ADCC capacity. Related references: PMID:24412922 PMID:25786175 PMID:29967280 PMID:9916693
• The receptor also contributes to the antitumor efficacy of therapeutic monoclonal antibodies by mediating ADCC of IgG-opsonized tumor cells, with enhanced activity observed for afucosylated IgG1 antibodies. Related references: PMID:27670158 PMID:34485821 PMID:28566370
• CD16 is primarily expressed on the surface of NK cells and a subset of circulating monocytes, and can also be found in a secreted form. Related references: PMID:2526846 PMID:27670158
• Additionally, CD16 is implicated in Dengue virus pathogenesis via antibody-dependent enhancement, where afucosylated anti-Dengue IgG1 antibodies facilitate viral entry into myeloid cells.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and phosphorylation, which regulate receptor function and signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 20-208) of human CD16, suggesting the antibody recognizes an epitope in this region; verify reactivity with both FCGR3A (P08637) and FCGR3B (O75015) isoforms if needed.
• For flow cytometry, utilize the biotin label with fluorophore-conjugated streptavidin secondary reagents; titrate both primary antibody and streptavidin conjugate to identify optimal signal-to-noise ratios.
• Include appropriate isotype controls and Fc blocking steps to minimize nonspecific binding, especially when analyzing Fc receptor-expressing cells such as NK cells and monocytes.
• Validate the antibody in the relevant sample system (e.g., human peripheral blood mononuclear cells) and consider fixation/permeabilization protocols carefully as they may affect epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CD16 antibody exemplifies our commitment to delivering high-quality reagents for immunodiagnostic and therapeutic monitoring applications. We offer validated antibody pairs, bulk ancillary reagents, and custom raw-material sourcing to support your NK cell function, ADCC, and Fc receptor biology research with reliable supply and expert guidance.